| RRC ID |
83779
|
| Author |
Takaoka M, Hiraki-Kajiyama T, Miyasaka N, Hino T, Kondo K, Yoshihara Y.
|
| Title |
Single-Cell RNA-Sequencing of Zebrafish Olfactory Epithelium Identifies Odor-Responsive Candidate Olfactory Receptors.
|
| Journal |
Genes Cells
|
| Abstract |
Single-cell RNA-sequencing (scRNA-seq) is a powerful method to comprehensively overlook gene expression profiles of individual cells in various tissues, providing fundamental datasets for classification of cell types and further functional analyses. Here we adopted scRNA-seq analysis for the zebrafish olfactory sensory neurons which respond to water-borne odorants and pheromones to elicit various behaviors crucial for survival and species preservation. Firstly, a single-cell dissociation procedure of the zebrafish olfactory rosettes was optimized by using cold-active protease, minimizing artifactual neuronal activation. Secondly, various cell types were classified into distinct clusters, based on the expressions of well-defined marker genes. Notably, we validated non-overlapping expressions of different families of olfactory receptors among the clusters of olfactory sensory neurons. Lastly, we succeeded in estimating candidate olfactory receptors responding to a particular odor stimulus by carefully scrutinizing correlated expressions of immediate early genes. Thus, scRNA-seq is a useful measure for the analysis of olfactory sensory neurons not only in classifying functional cell types but also in identifying olfactory receptor genes for given odorants and pheromones.
|
| Volume |
30(1)
|
| Pages |
e13191
|
| Published |
2025-1-1
|
| DOI |
10.1111/gtc.13191
|
| PMID |
39789807
|
| PMC |
PMC11718239
|
| MeSH |
Animals
Odorants*
Olfactory Mucosa* / metabolism
Olfactory Receptor Neurons* / metabolism
RNA-Seq / methods
Receptors, Odorant* / genetics
Receptors, Odorant* / metabolism
Sequence Analysis, RNA* / methods
Single-Cell Analysis* / methods
Transcriptome / genetics
Zebrafish* / genetics
|
| IF |
1.655
|
| Resource |
| Zebrafish |
SAGFF179A
Tg(UAS:GFP) |
