Reference - Detail
| RRC ID | 83822 |
|---|---|
| Author | Atsuta Y, Chen YC, Hattori Y, Takemoto T, Saito D. |
| Title | Generation of a transgenic chicken line with reporters for limb bud mesenchyme and apical ectodermal ridge cells. |
| Journal | Dev Biol |
| Abstract |
Cell type-specific reporter transgenic chicken lines are invaluable tools in developmental biology, allowing the visualization of dynamics and differentiation states of target cell types in living embryos. Here, we report the establishment of a new transgenic chicken line in which limb mesenchyme and apical ectodermal ridge (AER) cells are labeled with different fluorescent proteins in the embryos. The processes for generating the reporter line involved using tissue-specific promoters, the Tol2 transposon-mediated genomic integration, and clonal culture system of primordial germ cells. Employing the transgenic chickens would facilitate the detailed characterization of limb mesenchyme and AER cells. Thus, this reporter chicken line will be a powerful tool for advancing the study of vertebrate limb development. |
| Volume | 520 |
| Pages | 53-61 |
| Published | 2025-4-1 |
| DOI | 10.1016/j.ydbio.2025.01.003 |
| PII | S0012-1606(25)00009-0 |
| PMID | 39788366 |
| MeSH | Animals Animals, Genetically Modified* / embryology Chick Embryo Chickens* / genetics Ectoderm* / cytology Ectoderm* / embryology Ectoderm* / metabolism Genes, Reporter* Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Limb Buds* / embryology Limb Buds* / metabolism Mesoderm* / cytology Mesoderm* / embryology Mesoderm* / metabolism Promoter Regions, Genetic / genetics |
| IF | 2.896 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Bluesky |
| Total number of mentions | 9 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Chicken / Quail | |