論文 - 詳細
| RRC ID | 83850 |
|---|---|
| 著者 | Sipani R, Rawal Y, Barman J, Abburi P, Kurlawala V, Joshi R. |
| タイトル | Drosophila grainyhead gene and its neural stem cell specific enhancers show epigenetic synchrony in the cells of the central nervous system. |
| ジャーナル | Dev Biol |
| Abstract |
Enhancers are the epicentres of tissue-specific gene regulation. In this study, we have used the central nervous system (CNS) specific expression of the Drosophila grainyhead (grh) gene to make a case for deleting the enhancers in a sensitised background of other enhancer deletion, to functionally validate their role in tissue-specific gene regulation. We identified novel enhancers for grh and subsequently deleted two of them, to establish their collective importance in regulating grh expression in CNS. This showed that grh relies on multiple enhancers for its robust expression in neural stem cells (NSCs), with different combinations of enhancers playing a critical role in regulating its expression in various subset of these cells. We also found that these enhancers and the grh gene show epigenetic synchrony across the three cell types (NSCs, intermediate progenitors and neurons) of the developing CNS; and grh is not transcribed in intermediate progenitor cells, which inherits the Grh protein from the NSCs. We propose that this could be a general mechanism for regulating the expression of cell fate determinant protein in intermediate progenitor cells. Lastly, our results underline that enhancer redundancy results in phenotypic robustness in grh gene expression, which seems to be a consequence of the cumulative activity of multiple enhancers. |
| 巻・号 | 522 |
| ページ | 227-239 |
| 公開日 | 2025-6-1 |
| DOI | 10.1016/j.ydbio.2025.03.012 |
| PII | S0012-1606(25)00074-0 |
| PMID | 40154783 |
| MeSH | Animals Central Nervous System* / cytology Central Nervous System* / embryology Central Nervous System* / metabolism DNA-Binding Proteins* / genetics DNA-Binding Proteins* / metabolism Drosophila Proteins* / genetics Drosophila Proteins* / metabolism Drosophila melanogaster* / embryology Drosophila melanogaster* / genetics Enhancer Elements, Genetic* / genetics Epigenesis, Genetic* Gene Expression Regulation, Developmental Neural Stem Cells* / cytology Neural Stem Cells* / metabolism Transcription Factors* / genetics Transcription Factors* / metabolism |
| IF | 2.896 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.5 |
| リソース情報 | |
| ショウジョウバエ | |