| RRC ID |
83904
|
| Author |
Volkov LI, Ogawa Y, Somjee R, Vedder HE, Powell HE, Poria D, Meiselman S, Kefalov VJ, Corbo JC.
|
| Title |
Samd7 represses short-wavelength cone genes to preserve long-wavelength cone and rod photoreceptor identity.
|
| Journal |
Proc Natl Acad Sci U S A
|
| Abstract |
The role of transcription factors in photoreceptor gene regulation is fairly well understood, but knowledge of the cell-type-specific function of transcriptional cofactors remains incomplete. Here, we show that the transcriptional corepressor samd7 promotes rod differentiation and represses short-wavelength cone genes in long-wavelength cones in zebrafish. In samd7-/- retinas, red cones are transformed into hybrid red/ultraviolet (UV) cones, green cones are absent, the number of blue cones is approximately doubled, and the number of rods is greatly reduced. We also find that mouse Samd7 represses S-opsin expression in dorsal M-cones-analogous to its role in repressing UV cone genes in zebrafish red cones. Thus, samd7 plays a key role in ensuring appropriate patterns of gene expression in rods and cone subtypes of both zebrafish and mice.
|
| Volume |
121(47)
|
| Pages |
e2402121121
|
| Published |
2024-11-19
|
| DOI |
10.1073/pnas.2402121121
|
| PMID |
39531499
|
| PMC |
PMC11588049
|
| MeSH |
Animals
Cell Differentiation / genetics
Gene Expression Regulation
Mice
Mice, Knockout
Repressor Proteins / genetics
Repressor Proteins / metabolism
Retinal Cone Photoreceptor Cells* / metabolism
Retinal Rod Photoreceptor Cells* / metabolism
Zebrafish* / genetics
Zebrafish Proteins* / genetics
Zebrafish Proteins* / metabolism
|
| IF |
9.412
|
| Resource |
| Zebrafish |
Tg(zfSWS1-5.5A:EGFP)kj9
Tg(zfSWS2-3.5A:EGFP)kj11 / tkk008
Tg(rho:EGFP)ja2 |
