論文 - 詳細
| RRC ID | 83908 |
|---|---|
| 著者 | Li Y, Xu B, Jin M, Zhang H, Ren N, Hu J, He J. |
| タイトル | Homophilic interaction of cell adhesion molecule 3 coordinates retina neuroepithelial cell proliferation. |
| ジャーナル | J Cell Biol |
| Abstract |
Correct cell number generation is central to tissue development. However, in vivo roles of coordinated proliferation of individual neural progenitors in regulating cell numbers of developing neural tissues and the underlying molecular mechanism remain mostly elusive. Here, we showed that wild-type (WT) donor retinal progenitor cells (RPCs) generated significantly expanded clones in host retinae with G1-lengthening by p15 (cdkn2a/b) overexpression (p15+) in zebrafish. Further analysis showed that cell adhesion molecule 3 (cadm3) was reduced in p15+ host retinae, and overexpression of either full-length or ectodomains of Cadm3 in p15+ host retinae markedly suppressed the clonal expansion of WT donor RPCs. Notably, WT donor RPCs in retinae with cadm3 disruption recapitulated expanded clones that were found in p15+ retinae. More strikingly, overexpression of Cadm3 without extracellular ig1 domain in RPCs resulted in expanded clones and increased retinal total cell number. Thus, homophilic interaction of Cadm3 provides an intercellular mechanism underlying coordinated cell proliferation to ensure cell number homeostasis of the developing neuroepithelia. |
| 巻・号 | 222(6) |
| 公開日 | 2023-6-5 |
| DOI | 10.1083/jcb.202204098 |
| PII | 214005 |
| PMID | 37022761 |
| PMC | PMC10082328 |
| MeSH | Animals Cell Adhesion Molecules* / genetics Cell Adhesion Molecules* / metabolism Cell Differentiation / physiology Cell Proliferation Neuroepithelial Cells / metabolism Retina* / metabolism Zebrafish* / genetics Zebrafish* / metabolism Zebrafish Proteins* / genetics Zebrafish Proteins* / metabolism |
| IF | 8.811 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 27 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | Tg(EF1α:mKO2-zCdt1(1/190))rw0405b Tg(EF1α:mAG-zGem(1/100))rw0410h |