| RRC ID |
83921
|
| Author |
Skribbe M, Soneson C, Stadler MB, Schwaiger M, Suma Sreechakram VN, Iesmantavicius V, Hess D, Moreno EPF, Braun S, Seebacher J, Smallwood SA, Bühler M.
|
| Title |
A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
|
| Journal |
Mol Cell
|
| Abstract |
Transcription factors (TFs) are key regulators of gene expression, yet many of their targets and modes of action remain unknown. In Schizosaccharomyces pombe, one-third of TFs are solely homology predicted, with few experimentally validated. We created a comprehensive library of 89 endogenously tagged S. pombe TFs, mapping their protein and chromatin interactions using immunoprecipitation-mass spectrometry and chromatin immunoprecipitation sequencing. Our study identified protein interactors for half the TFs, with over a quarter potentially forming stable complexes. We discovered DNA-binding sites for most TFs across 2,027 unique genomic regions, revealing motifs for 38 TFs and uncovering a complex network of extensive TF cross- and autoregulation. Characterization of the largest TF family revealed conserved DNA sequence preferences but diverse binding patterns and identified a repressive heterodimer, Ntu1/Ntu2, linked to perinuclear gene localization. Our TFexplorer webtool makes all data interactively accessible, offering insights into TF interactions and regulatory mechanisms with broad biological relevance.
|
| Volume |
85(7)
|
| Pages |
1426-1444.e8
|
| Published |
2025-4-3
|
| DOI |
10.1016/j.molcel.2025.01.032
|
| PII |
S1097-2765(25)00099-1
|
| PMID |
40015273
|
| MeSH |
Binding Sites
Chromatin* / genetics
Chromatin* / metabolism
Chromatin Immunoprecipitation Sequencing
Gene Expression Regulation, Fungal
Protein Binding
Schizosaccharomyces* / genetics
Schizosaccharomyces* / metabolism
Schizosaccharomyces pombe Proteins* / genetics
Schizosaccharomyces pombe Proteins* / metabolism
Transcription Factors* / genetics
Transcription Factors* / metabolism
|
| IF |
15.584
|
| Resource |
| Yeast |
FY38775 |
