論文 - 詳細
| RRC ID | 83921 |
|---|---|
| 著者 | Skribbe M, Soneson C, Stadler MB, Schwaiger M, Suma Sreechakram VN, Iesmantavicius V, Hess D, Moreno EPF, Braun S, Seebacher J, Smallwood SA, Bühler M. |
| タイトル | A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin. |
| ジャーナル | Mol Cell |
| Abstract |
Transcription factors (TFs) are key regulators of gene expression, yet many of their targets and modes of action remain unknown. In Schizosaccharomyces pombe, one-third of TFs are solely homology predicted, with few experimentally validated. We created a comprehensive library of 89 endogenously tagged S. pombe TFs, mapping their protein and chromatin interactions using immunoprecipitation-mass spectrometry and chromatin immunoprecipitation sequencing. Our study identified protein interactors for half the TFs, with over a quarter potentially forming stable complexes. We discovered DNA-binding sites for most TFs across 2,027 unique genomic regions, revealing motifs for 38 TFs and uncovering a complex network of extensive TF cross- and autoregulation. Characterization of the largest TF family revealed conserved DNA sequence preferences but diverse binding patterns and identified a repressive heterodimer, Ntu1/Ntu2, linked to perinuclear gene localization. Our TFexplorer webtool makes all data interactively accessible, offering insights into TF interactions and regulatory mechanisms with broad biological relevance. |
| 巻・号 | 85(7) |
| ページ | 1426-1444.e8 |
| 公開日 | 2025-4-3 |
| DOI | 10.1016/j.molcel.2025.01.032 |
| PII | S1097-2765(25)00099-1 |
| PMID | 40015273 |
| MeSH | Binding Sites Chromatin* / genetics Chromatin* / metabolism Chromatin Immunoprecipitation Sequencing Gene Expression Regulation, Fungal Protein Binding Schizosaccharomyces* / genetics Schizosaccharomyces* / metabolism Schizosaccharomyces pombe Proteins* / genetics Schizosaccharomyces pombe Proteins* / metabolism Transcription Factors* / genetics Transcription Factors* / metabolism |
| IF | 15.584 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 37 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | FY38775 FY49691-FY49780 |