論文 - 詳細
| RRC ID | 83965 |
|---|---|
| 著者 | Ouzounidis VR, Green M, van Capelle CC, Gebhardt C, Crellin H, Finlayson C, Prevo B, Cheerambathur DK. |
| タイトル | The outer kinetochore components KNL-1 and Ndc80 complex regulate axon and neuronal cell body positioning in the C. elegans nervous system. |
| ジャーナル | Mol Biol Cell |
| Abstract |
The KMN (Knl1/Mis12/Ndc80) network at the kinetochore, primarily known for its role in chromosome segregation, has been shown to be repurposed during neurodevelopment. Here, we investigate the underlying neuronal mechanism and show that the KMN network promotes the proper axonal organization within the C. elegans head nervous system. Postmitotic degradation of KNL-1, which acts as a scaffold for signaling and has microtubule-binding activities at the kinetochore, led to disorganized ganglia and aberrant placement and organization of axons in the nerve ring - an interconnected axonal network. Through gene-replacement approaches, we demonstrate that the signaling motifs within KNL-1, responsible for recruiting protein phosphatase 1, and activating the spindle assembly checkpoint are required for neurodevelopment. Interestingly, while the microtubule-binding activity is crucial to KMN's neuronal function, microtubule dynamics and organization were unaffected in the absence of KNL-1. Instead, the NDC-80 microtubule-binding mutant displayed notable defects in axon bundling during nerve ring formation, indicating its role in facilitating axon-axon contacts. Overall, these findings provide evidence for a noncanonical role for the KMN network in shaping the structure and connectivity of the nervous system in C. elegans during brain development. |
| 巻・号 | 35(6) |
| ページ | ar83 |
| 公開日 | 2024-6-1 |
| DOI | 10.1091/mbc.E23-08-0325 |
| PMID | 38656792 |
| PMC | PMC11238089 |
| MeSH | Animals Axons* / metabolism Axons* / physiology Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Chromosome Segregation Cytoskeletal Proteins / metabolism Kinetochores* / metabolism Microtubule-Associated Proteins* / metabolism Microtubules* / metabolism Nervous System / metabolism Neurons* / metabolism Signal Transduction Spindle Apparatus / metabolism |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm5271 |