論文 - 詳細
| RRC ID | 83998 |
|---|---|
| 著者 | Hellinga JR, Krücken J, Schulenburg H, von Samson-Himmelstjerna G. |
| タイトル | Use of Viscous medium to study anthelmintic drug action in Caenorhabditis elegans. |
| ジャーナル | Sci Rep |
| Abstract |
Caenorhabditis elegans is an appealing tool for experimental evolution and for working with antiparasitic drugs, from understanding the molecular mechanisms of drug action and resistance to uncover new drug targets. We present a new methodology for studying the impact of antiparasitic drugs in C. elegans. Viscous medium was initially designed for C. elegans maintenance during long-term evolution experiments. Viscous medium provides a less structured environment than the standard nematode growth media agar, yet the bacteria food source remains suspended. Further, the Viscous medium offers the worm population enough support to move freely, mate, and reproduce at a rate comparable to standard agar cultures. Here, the Viscous medium was adapted for use in antiparasitic research. We observed a similar sensitivity of C. elegans to anthelmintic drugs as in standard liquid media and statistical difference to the standard agar media through a larval development assay. Using Viscous medium in C. elegans studies will considerably improve antiparasitic resistance research, and this medium could be used in studies aimed at understanding long-term multigenerational drug activity. |
| 巻・号 | 14(1) |
| ページ | 12756 |
| 公開日 | 2024-6-4 |
| DOI | 10.1038/s41598-024-63090-z |
| PII | 10.1038/s41598-024-63090-z |
| PMID | 38830930 |
| PMC | PMC11148144 |
| MeSH | Agar Animals Anthelmintics* / pharmacology Caenorhabditis elegans* / drug effects Caenorhabditis elegans* / growth & development Culture Media* / chemistry Drug Resistance / drug effects Larva / drug effects Viscosity |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm830 |