論文 - 詳細
| RRC ID | 84001 |
|---|---|
| 著者 | Yu X, Zhou J, Ye W, Xu J, Li R, Huang L, Chai Y, Wen M, Xu S, Zhou Y. |
| タイトル | Time-course swRNA-seq uncovers a hierarchical gene regulatory network in controlling the response-repair-remodeling after wounding. |
| ジャーナル | Commun Biol |
| Abstract |
Wounding initiates intricate responses crucial for tissue repair and regeneration. Yet, the gene regulatory networks governing wound healing remain poorly understood. Here, employing single-worm RNA sequencing (swRNA-seq) across 12 time-points, we delineated a three-stage wound repair process in C. elegans: response, repair, and remodeling. Integrating diverse datasets, we constructed a dynamic regulatory network comprising 241 transcription regulators and their inferred targets. We identified potentially seven autoregulatory TFs and five cross-autoregulatory loops involving pqm-1 and jun-1. We revealed that TFs might interact with chromatin factors and form TF-TF combinatory modules via intrinsically disordered regions to enhance response robustness. We experimentally validated six regulators functioning in transcriptional and translocation-dependent manners. Notably, nhr-76, daf-16, nhr-84, and oef-1 are potentially required for efficient repair, while elt-2 may act as an inhibitor. These findings elucidate transcriptional responses and hierarchical regulatory networks during C. elegans wound repair, shedding light on mechanisms underlying tissue repair and regeneration. |
| 巻・号 | 7(1) |
| ページ | 694 |
| 公開日 | 2024-6-6 |
| DOI | 10.1038/s42003-024-06352-w |
| PII | 10.1038/s42003-024-06352-w |
| PMID | 38844830 |
| PMC | PMC11156874 |
| MeSH | Animals Caenorhabditis elegans* / genetics Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Gene Expression Regulation Gene Regulatory Networks* Sequence Analysis, RNA Transcription Factors / genetics Transcription Factors / metabolism Wound Healing* / genetics |
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オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm4063 |