| RRC ID |
84001
|
| Author |
Yu X, Zhou J, Ye W, Xu J, Li R, Huang L, Chai Y, Wen M, Xu S, Zhou Y.
|
| Title |
Time-course swRNA-seq uncovers a hierarchical gene regulatory network in controlling the response-repair-remodeling after wounding.
|
| Journal |
Commun Biol
|
| Abstract |
Wounding initiates intricate responses crucial for tissue repair and regeneration. Yet, the gene regulatory networks governing wound healing remain poorly understood. Here, employing single-worm RNA sequencing (swRNA-seq) across 12 time-points, we delineated a three-stage wound repair process in C. elegans: response, repair, and remodeling. Integrating diverse datasets, we constructed a dynamic regulatory network comprising 241 transcription regulators and their inferred targets. We identified potentially seven autoregulatory TFs and five cross-autoregulatory loops involving pqm-1 and jun-1. We revealed that TFs might interact with chromatin factors and form TF-TF combinatory modules via intrinsically disordered regions to enhance response robustness. We experimentally validated six regulators functioning in transcriptional and translocation-dependent manners. Notably, nhr-76, daf-16, nhr-84, and oef-1 are potentially required for efficient repair, while elt-2 may act as an inhibitor. These findings elucidate transcriptional responses and hierarchical regulatory networks during C. elegans wound repair, shedding light on mechanisms underlying tissue repair and regeneration.
|
| Volume |
7(1)
|
| Pages |
694
|
| Published |
2024-6-6
|
| DOI |
10.1038/s42003-024-06352-w
|
| PII |
10.1038/s42003-024-06352-w
|
| PMID |
38844830
|
| PMC |
PMC11156874
|
| MeSH |
Animals
Caenorhabditis elegans* / genetics
Caenorhabditis elegans Proteins* / genetics
Caenorhabditis elegans Proteins* / metabolism
Gene Expression Regulation
Gene Regulatory Networks*
Sequence Analysis, RNA
Transcription Factors / genetics
Transcription Factors / metabolism
Wound Healing* / genetics
|
| Resource |
| C.elegans |
tm4063 |
