論文 - 詳細
| RRC ID | 84025 |
|---|---|
| 著者 | Xu Z, Wang Z, Wang L, Qi YB. |
| タイトル | Essential function of transmembrane transcription factor MYRF in promoting transcription of miRNA lin-4 during C. elegans development. |
| ジャーナル | Elife |
| Abstract |
Precise developmental timing control is essential for organism formation and function, but its mechanisms are unclear. In C. elegans, the microRNA lin-4 critically regulates developmental timing by post-transcriptionally downregulating the larval-stage-fate controller LIN-14. However, the mechanisms triggering the activation of lin-4 expression toward the end of the first larval stage remain unknown. We demonstrate that the transmembrane transcription factor MYRF-1 is necessary for lin-4 activation. MYRF-1 is initially localized on the cell membrane, and its increased cleavage and nuclear accumulation coincide with lin-4 expression timing. MYRF-1 regulates lin-4 expression cell-autonomously and hyperactive MYRF-1 can prematurely drive lin-4 expression in embryos and young first-stage larvae. The tandem lin-4 promoter DNA recruits MYRF-1GFP to form visible loci in the nucleus, suggesting that MYRF-1 directly binds to the lin-4 promoter. Our findings identify a crucial link in understanding developmental timing regulation and establish MYRF-1 as a key regulator of lin-4 expression. |
| 巻・号 | 12 |
| 公開日 | 2024-7-4 |
| DOI | 10.7554/eLife.89903 |
| PII | 89903 |
| PMID | 38963411 |
| PMC | PMC11223767 |
| MeSH | Animals Caenorhabditis elegans* / genetics Caenorhabditis elegans* / growth & development Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Cell Nucleus / metabolism Gene Expression Regulation, Developmental* Membrane Proteins / genetics Membrane Proteins / metabolism MicroRNAs* / genetics MicroRNAs* / metabolism Promoter Regions, Genetic Transcription Factors* / genetics Transcription Factors* / metabolism Transcription, Genetic |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tmIs1028 |