論文 - 詳細
| RRC ID | 84041 |
|---|---|
| 著者 | Kelley LH, Caldas IV, Sullenberger MT, Yongblah KE, Niazi AM, Iyer A, Li Y, Tran PM, Valen E, Ahmed-Braimah YH, Maine EM. |
| タイトル | Poly(U) polymerase activity in Caenorhabditis elegans regulates abundance and tailing of sRNA and mRNA. |
| ジャーナル | Genetics |
| Abstract |
Terminal nucleotidyltransferases add nucleotides to the 3' end of RNA to modify their stability and function. In Caenorhabditis elegans, the terminal uridyltransferases/poly(U) polymerases PUP-1 (aka CID-1, CDE-1), PUP-2, and PUP-3 affect germline identity, survival, and development. Here, we identify small RNA (sRNA) and mRNA targets of these PUPs and of a fourth predicted poly(U) polymerase, F43E2.1/PUP-4. Using genetic and RNA sequencing approaches, we identify RNA targets of each PUP and the U-tail frequency and length of those targets. At the whole organism level, PUP-1 is responsible for most sRNA U-tailing, and other PUPs contribute to modifying discrete subsets of sRNAs. Moreover, the expression of PUP-2, PUP-3, and especially PUP-4 limits uridylation on some sRNAs. The relationship between uridylation status and sRNA abundance suggests that U-tailing can have a negative or positive effect on abundance depending on context. sRNAs modified by PUP activity primarily target mRNAs that are ubiquitously expressed or most highly expressed in the germline. mRNA data obtained with a Nanopore-based method reveal that the addition of U-tails to nonadenylated mRNA is substantially reduced in the absence of PUP-3. Overall, this work identifies PUP RNA targets, defines the effect of uridylation loss on RNA abundance, and reveals the complexity of PUP regulation in C. elegans development. |
| 巻・号 | 228(2) |
| 公開日 | 2024-10-7 |
| DOI | 10.1093/genetics/iyae120 |
| PII | 7721997 |
| PMID | 39067069 |
| PMC | PMC11457939 |
| MeSH | Animals Caenorhabditis elegans* / genetics Caenorhabditis elegans* / growth & development Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism RNA Nucleotidyltransferases* / genetics RNA Nucleotidyltransferases* / metabolism RNA, Messenger* / genetics RNA, Messenger* / metabolism RNA, Small Untranslated* / genetics RNA, Small Untranslated* / metabolism |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm5089 tm4344 tm1021 |