Reference - Detail
| RRC ID | 84052 |
|---|---|
| Author | Cao W, Fan Q, Amparado G, Begic D, Godini R, Gopal S, Pocock R. |
| Title | A nucleic acid binding protein map of germline regulation in Caenorhabditis elegans. |
| Journal | Nat Commun |
| Abstract |
Fertility requires the faithful proliferation of germ cells and their differentiation into gametes. Controlling these cellular states demands precise timing and expression of gene networks. Nucleic acid binding proteins (NBPs) play critical roles in gene expression networks that influence germ cell development. There has, however, been no functional analysis of the entire NBP repertoire in controlling in vivo germ cell development. Here, we analyzed germ cell states and germline architecture to systematically investigate the function of 364 germline-expressed NBPs in the Caenorhabditis elegans germ line. Using germline-specific knockdown, automated germ cell counting, and high-content analysis of germ cell nuclei and plasma membrane organization, we identify 156 NBPs with discrete autonomous germline functions. By identifying NBPs that control the germ cell cycle, proliferation, differentiation, germline structure and fertility, we have created an atlas for mechanistic dissection of germ cell behavior and gamete production. |
| Volume | 15(1) |
| Pages | 6884 |
| Published | 2024-8-11 |
| DOI | 10.1038/s41467-024-51212-0 |
| PII | 10.1038/s41467-024-51212-0 |
| PMID | 39128930 |
| PMC | PMC11317507 |
| MeSH | Animals Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Cell Cycle / genetics Cell Differentiation / genetics Cell Proliferation / genetics DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Fertility / genetics Gene Expression Regulation, Developmental Gene Regulatory Networks Germ Cells* / metabolism Male RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism |
| Resource | |
| C.elegans | tm356 tm3541 tm1083 tm3052 tm7936 tm3112 tm4799 tm1736 tm4302 tm7982 tm8341 tm2539 tm769 tm529 tm4566 tm611 tm4820 tm574 tm289 tm514 tm2975 tm4926 tm4919 tm2359 tm4567 tm2349 tm5029 tm4385 tm4827 tm528 tm586 tm1710 tm1021 tm4597 tm4568 tm3586 tm1976 tm1286 tm4795 tm4436 tm4787 tm936 tm932 tm4914 tm282 tm4769 tm4372 tm1423 |