論文 - 詳細
| RRC ID | 84087 |
|---|---|
| 著者 | Zupančič M, Keimpema E, Tretiakov EO, Eder SJ, Lev I, Englmaier L, Bhandari P, Fietz SA, Härtig W, Renaux E, Villunger A, Hökfelt T, Zimmer M, Clotman F, Harkany T. |
| タイトル | Concerted transcriptional regulation of the morphogenesis of hypothalamic neurons by ONECUT3. |
| ジャーナル | Nat Commun |
| Abstract |
Acquisition of specialized cellular features is controlled by the ordered expression of transcription factors (TFs) along differentiation trajectories. Here, we find a member of the Onecut TF family, ONECUT3, expressed in postmitotic neurons that leave their Ascl1+/Onecut1/2+ proliferative domain in the vertebrate hypothalamus to instruct neuronal differentiation. We combined single-cell RNA-seq and gain-of-function experiments for gene network reconstruction to show that ONECUT3 affects the polarization and morphogenesis of both hypothalamic GABA-derived dopamine and thyrotropin-releasing hormone (TRH)+ glutamate neurons through neuron navigator-2 (NAV2). In vivo, siRNA-mediated knockdown of ONECUT3 in neonatal mice reduced NAV2 mRNA, as well as neurite complexity in Onecut3-containing neurons, while genetic deletion of Onecut3/ceh-48 in C. elegans impaired neurocircuit wiring, and sensory discrimination-based behaviors. Thus, ONECUT3, conserved across neuronal subtypes and many species, underpins the polarization and morphological plasticity of phenotypically distinct neurons that descend from a common pool of Ascl1+ progenitors in the hypothalamus. |
| 巻・号 | 15(1) |
| ページ | 8631 |
| 公開日 | 2024-10-5 |
| DOI | 10.1038/s41467-024-52762-z |
| PII | 10.1038/s41467-024-52762-z |
| PMID | 39366958 |
| PMC | PMC11452682 |
| MeSH | Animals Basic Helix-Loop-Helix Transcription Factors / genetics Basic Helix-Loop-Helix Transcription Factors / metabolism Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Cell Differentiation / genetics Female Hypothalamus* / cytology Hypothalamus* / metabolism Male Mice Mice, Inbred C57BL Morphogenesis* / genetics Neurogenesis / genetics Neurons* / cytology Neurons* / metabolism Transcription Factors* / genetics Transcription Factors* / metabolism |
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オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm237 |