論文 - 詳細
| RRC ID | 84143 |
|---|---|
| 著者 | Gold AL, Hurlock ME, Guevara AM, Isenberg LYZ, Kim Y. |
| タイトル | Identification of the Polo-like kinase substrate required for homologous synapsis. |
| ジャーナル | J Cell Biol |
| Abstract |
The synaptonemal complex (SC) is a zipper-like protein structure that aligns homologous chromosome pairs and regulates recombination during meiosis. Despite its conserved appearance and function, how synapsis occurs between chromosome axes remains elusive. Here, we demonstrate that Polo-like kinases (PLKs) phosphorylate a single conserved residue in the disordered C-terminal tails of two paralogous SC subunits, SYP-5 and SYP-6, to establish an electrostatic interface between the SC central region and chromosome axes in C. elegans. While SYP-5/6 phosphorylation is dispensable for the ability of SC proteins to self-assemble, local phosphorylation by PLKs at the pairing center is crucial for SC elongation between homologous chromosome axes. Additionally, SYP-5/6 phosphorylation is essential for asymmetric SC disassembly and proper PLK-2 localization after crossover designation, which drives chromosome remodeling required for homolog separation during meiosis I. This work identifies a key regulatory mechanism by which localized PLK activity mediates the SC-axis interaction through phosphorylation of SYP-5/6, coupling synapsis initiation to homolog pairing. |
| 巻・号 | 224(3) |
| 公開日 | 2025-3-3 |
| DOI | 10.1083/jcb.202408092 |
| PII | 277162 |
| PMID | 39680026 |
| PMC | PMC11648704 |
| MeSH | Animals Caenorhabditis elegans* / enzymology Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Chromosome Pairing* / genetics Meiosis* / genetics Phosphorylation Polo-like Kinases Protein Serine-Threonine Kinases* / genetics Protein Serine-Threonine Kinases* / metabolism Synaptonemal Complex* / genetics Synaptonemal Complex* / metabolism |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 20 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1395 tm3298 |