論文 - 詳細
| RRC ID | 84144 |
|---|---|
| 著者 | Kurashina M, Snow AW, Mizumoto K. |
| タイトル | A modular system to label endogenous presynaptic proteins using split fluorophores in Caenorhabditis elegans. |
| ジャーナル | Genetics |
| Abstract |
Visualizing the subcellular localization of presynaptic proteins with fluorescent proteins is a powerful tool to dissect the genetic and molecular mechanisms underlying synapse formation and patterning in live animals. Here, we utilize split green and red fluorescent proteins to visualize the localization of endogenously expressed presynaptic proteins at a single-neuron resolution in Caenorhabditis elegans. By using CRISPR/Cas9 genome editing, we generated a collection of C. elegans strains in which endogenously expressed presynaptic proteins (RAB-3/Rab3, SNG-1/Synaptogyrin, CLA-1/Piccolo, SYD-2/Liprin-α, UNC-10/RIM, RIMB-1/RIM-BP, and ELKS-1/ELKS) are tagged with tandem repeats of GFP11 and/or wrmScarlet11. We show that the expression of GFP1-10 and wrmScarlet1-10 under neuron-specific promoters can robustly label presynaptic proteins in different neuron types. We believe that the combination of our knock-in strains and GFP1-10 and wrmScarlet1-10 plasmids is a versatile modular system useful for neuroscientists to examine the localization of endogenous presynaptic proteins in any neuron type in C. elegans. |
| 巻・号 | 229(3) |
| 公開日 | 2025-3-17 |
| DOI | 10.1093/genetics/iyae214 |
| PII | 7930300 |
| PMID | 39708832 |
| PMC | PMC11912834 |
| MeSH | Animals CRISPR-Cas Systems Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Gene Editing Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Luminescent Proteins* / genetics Luminescent Proteins* / metabolism Neurons / metabolism Presynaptic Terminals* / metabolism Red Fluorescent Protein |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 15 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3275 |