| Abstract |
Free radicals are believed to play a secondary role in the cell death cascade associated with various diseases. In Huntington's disease (HD), the aggregation of polyglutamine (PolyQ) not only contributes to the disease but also elevates free radical levels. However, measuring free radicals is difficult due to their short lifespan and limited diffusion range. Here, a quantum sensing technique (T1 relaxometry) is used that involves fluorescent nanodiamonds (FND). Nitrogen vacancy (NV) centers within these nanodiamonds change their optical properties in response to magnetic noise, which allows detecting the unpaired electron from free radicals. This method is used to monitor the production of free radicals inside Caenorhabditis elegans models of Huntington's disease in vivo and in real-time. To investigate if radical generation occurs near polyglutamine expansions, a strain expressing Q40 yellow fluorescent protein (Q40::YFP, polyglutamine expansion overexpressed in the muscle) is used. By applying T1 relaxometry on FNDs in the body wall muscle, it is found that the production of free radicals significantly increase when PolyQ is expressed there (compared to the FNDs in intestine). The technique demonstrates the submicrometer localization of free radical information in living animals and direct measurement of their level, which may reveal the relation between oxidative stress and Huntington's disease.
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