Reference - Detail
| RRC ID | 84217 |
|---|---|
| Author | Stubbe FX, Ponsard P, Steiner FA, Hermand D. |
| Title | SSUP-72/PINN-1 coordinates RNA-polymerase II 3' pausing and developmental gene expression in C. elegans. |
| Journal | Nat Commun |
| Abstract |
During exit from Caenorhabditis elegans (C. elegans) L1 developmental arrest, a network of growth- and developmental genes is activated, many of which are organized into operons where transcriptional termination is uncoupled from mRNA 3'-end processing. CDK-12-mediated Pol II CTD S2 phosphorylation enhances SL2 trans-splicing at downstream operonic genes, preventing premature termination and ensuring proper gene expression for developmental progression. Using a genetic screen, we identified the SSUP-72/PINN-1 module as a suppressor of defects induced by CDK-12 inhibition. Loss of SSUP-72/PINN-1 bypasses the requirement for CDK-12 in post-embryonic development. Genome-wide analyses reveal that SSUP-72, a CTD S5P phosphatase, affects Pol II 3' pausing and regulates intra-operon termination. Our findings establish SSUP-72/PINN-1 as a key regulator of Pol II dynamics, coordinating operonic gene expression and growth during C. elegans post-embryonic development. |
| Volume | 16(1) |
| Pages | 2624 |
| Published | 2025-3-17 |
| DOI | 10.1038/s41467-025-57847-x |
| PII | 10.1038/s41467-025-57847-x |
| PMID | 40097442 |
| PMC | PMC11914089 |
| MeSH | Animals Caenorhabditis elegans* / genetics Caenorhabditis elegans* / growth & development Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Gene Expression Regulation, Developmental* Phosphoprotein Phosphatases / genetics Phosphoprotein Phosphatases / metabolism Phosphorylation RNA Polymerase II* / genetics RNA Polymerase II* / metabolism Trans-Splicing |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm2304 tm2235 tm3864 |