論文 - 詳細
| RRC ID | 84218 |
|---|---|
| 著者 | Harvey LM, Frédérick PM, Gudipati RK, Michaud P, Houle F, Young D, Desbiens C, Ladouceur S, Dufour A, Großhans H, Simard MJ. |
| タイトル | Dipeptidyl peptidase DPF-3 is a gatekeeper of microRNA Argonaute compensation in animals. |
| ジャーナル | Nat Commun |
| Abstract |
MicroRNAs (miRNAs) are essential regulators involved in multiple biological processes. To achieve their gene repression function, they are loaded in miRNA-specific Argonautes to form the miRNA-induced silencing complex (miRISC). Mammals and C. elegans possess more than one paralog of miRNA-specific Argonautes, but the dynamic between them remains unclear. Here, we report the conserved dipeptidyl peptidase DPF-3 as an interactor of the miRNA-specific Argonaute ALG-1 in C. elegans. Knockout of dpf-3 increases ALG-2 levels and miRISC formation in alg-1 loss-of-function animals, thereby compensating for ALG-1 loss and rescuing miRNA-related defects observed. DPF-3 can cleave an ALG-2 N-terminal peptide in vitro but does not appear to rely on this catalytic activity to regulate ALG-2 in vivo. This study uncovers the importance of DPF-3 in the miRNA pathway and provides insights into how multiple miRNA Argonautes contribute to achieving proper miRNA-mediated gene regulation in animals. |
| 巻・号 | 16(1) |
| ページ | 2738 |
| 公開日 | 2025-3-20 |
| DOI | 10.1038/s41467-025-58141-6 |
| PII | 10.1038/s41467-025-58141-6 |
| PMID | 40108168 |
| PMC | PMC11923051 |
| MeSH | Animals Argonaute Proteins* / genetics Argonaute Proteins* / metabolism Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Dipeptidyl-Peptidases and Tripeptidyl-Peptidases* / genetics Dipeptidyl-Peptidases and Tripeptidyl-Peptidases* / metabolism Gene Expression Regulation MicroRNAs* / genetics MicroRNAs* / metabolism RNA-Binding Proteins RNA-Induced Silencing Complex / genetics RNA-Induced Silencing Complex / metabolism |
| リソース情報 | |
| 線虫 | tm1120 tm492 |