RRC ID 84225
Author Sojka SE, Ezak MJ, Polk EA, Bischer AP, Neyland KE, Wojtovich AP, Ferkey DM.
Title An Extensive Gap Junction Neural Network Modulates Caenorhabditis elegans Aversive Behavior.
Journal Genes (Basel)
Abstract BACKGROUND/OBJECTIVES:Caenorhabditis elegans rely on sensory perception of environmental cues for survival in their native soil and compost habitats. These cues provide information about nutrient availability, mating partners, or predatory and hazardous beacons. In C. elegans, the two bilaterally-symmetric head sensory neurons termed ASH are the main detectors of aversive nociceptive signals. Through their downstream connections in the nervous system, ASH activation causes the animal to initiate backward locomotion to escape and avoid the harmful stimulus. Modulation of avoidance behavior allows for situation-appropriate sensitivity and response to stimuli. We previously reported a role for gap junctions in the transport of regulatory cGMP to the ASHs where it functions to dampen avoidance responses.
METHODS:Here, we used genetic mutants and a combination of cell-selective rescue and knockdown experiments to identify gap junction proteins (innexins) involved in modulating ASH-mediated nociceptive behavioral responses.
RESULTS:We have characterized six additional C. elegans innexins that have overlapping and distinct roles within this regulatory network: INX-7, INX-15, INX-16, INX-17, UNC-7, and UNC-9.
CONCLUSIONS:This work expands our understanding of the extent to which ASH sensitivity can be tuned in a non-cell-autonomous manner.
Volume 16(3)
Published 2025-2-23
DOI 10.3390/genes16030260
PII genes16030260
PMID 40149412
PMC PMC11941935
MeSH Animals Avoidance Learning / physiology Behavior, Animal / physiology Caenorhabditis elegans* / genetics Caenorhabditis elegans* / physiology Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Connexins / genetics Connexins / metabolism Gap Junctions* / metabolism Gap Junctions* / physiology Locomotion Membrane Proteins Nerve Net / metabolism Nerve Net / physiology Sensory Receptor Cells / metabolism Sensory Receptor Cells / physiology
Resource
C.elegans tm3292