Reference - Detail
| RRC ID | 84240 |
|---|---|
| Author | Ujie Y, Saito S, Fukaya K, Urabe D, Yaguchi T, Arai MA. |
| Title | Aspergillus terreus IFM 65899-THP-1 cells interaction triggers production of the natural product butyrolactone Ia, an immune suppressive compound. |
| Journal | Sci Rep |
| Abstract |
We focused on the possibility that pathogenic microorganisms might produce immune suppressors to evade the action of immune cells. Based on this possibility, we have recently developed new co-culture method of pathogenic actinomyces and immune cells, however, the interaction mechanism between pathogens and cells was still unclear. In this report, co-culturing pathogenic fungi and immune cells were investigated. Pathogenic fungus Aspergillus terreus IFM 65899 and THP-1 cells were co-cultured and isolated a co-culture specific compound, butyrolactone Ia (1). 1 inhibits the production of nitric oxide by RAW264 cells and exhibits regulatory effects on autophagy, suggesting 1 plays a defensive role in the response of A. terreus IFM 65899 to immune cells. Furthermore, dialysis experiments and micrographs indicated that "physical interaction" between A. terreus IFM 65899 and THP-1 cells may be required for the production of 1. This is the first report of co-culture method of fungi with immune cells and its interaction mechanism. |
| Volume | 14(1) |
| Pages | 28278 |
| Published | 2024-11-16 |
| DOI | 10.1038/s41598-024-79837-7 |
| PII | 10.1038/s41598-024-79837-7 |
| PMID | 39550448 |
| PMC | PMC11569209 |
| MeSH | 4-Butyrolactone* / analogs & derivatives 4-Butyrolactone* / metabolism Animals Aspergillus* / metabolism Autophagy Biological Products / pharmacology Coculture Techniques* Humans Mice Nitric Oxide* / metabolism RAW 264.7 Cells THP-1 Cells |
| IF | 3.998 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Pathogenic eukaryotic microorganisms | Aspergillus terreus IFM 65899 |
| Human and Animal Cells | RAW 264(RCB0535) |