| RRC ID |
84305
|
| Author |
Frey T, Murakami T, Maki K, Kawaue T, Tani N, Sugai A, Nakazawa N, Ishiguro KI, Adachi T, Kengaku M, Ohki K, Gotoh Y, Kishi Y.
|
| Title |
Age-associated reduction of nuclear shape dynamics in excitatory neurons of the visual cortex.
|
| Journal |
Aging Cell
|
| Abstract |
Neurons decline in their functionality over time, and age-related neuronal alterations are associated with phenotypes of neurodegenerative diseases. In nonneural tissues, an infolded nuclear shape has been proposed as a hallmark of aged cells and neurons with infolded nuclei have also been reported to be associated with neuronal activity. Here, we performed time-lapse imaging in the visual cortex of Nex-Cre;SUN1-GFP mice. Nuclear infolding was observed within 10 min of stimulation in young nuclei, while the aged nuclei were already infolded pre-stimulation and showed reduced dynamics of the morphology. In young nuclei, the depletion of the stimuli restored the nucleus to a spherical shape and reduced the dynamic behavior, suggesting that nuclear infolding is a reversible process. We also found the aged nucleus to be stiffer than the young one, further relating to the age-associated loss of nuclear shape dynamics. We reveal temporal changes in the nuclear shape upon external stimulation and observe that these morphological dynamics decrease with age.
|
| Volume |
22(9)
|
| Pages |
e13925
|
| Published |
2023-9-1
|
| DOI |
10.1111/acel.13925
|
| PMID |
37476844
|
| PMC |
PMC10497821
|
| MeSH |
Animals
Mice
Neurons*
Visual Cortex* / physiology
|
| IF |
7.238
|
| Resource |
| Aged mice |
加齢マウス |
