| RRC ID |
84317
|
| 著者 |
Lee JY, Yang YJ, So J, Kim S, Cho K.
|
| タイトル |
Allelic Analysis of the Gli-B1 Locus in Hexaploid Wheat Using Reverse-Phase-Ultra-Performance Liquid Chromatography.
|
| ジャーナル |
Molecules
|
| Abstract |
Wheat (Triticum aestivum L.) omega-5 gliadin, a major allergen responsible for wheat-dependent exercise-induced anaphylaxis in humans, is encoded by genes located at the Gli-B1 locus on chromosome 1B, which exhibits genetic polymorphism. Gli-B1 alleles have generally been identified based on the electrophoretic mobilities of the encoded gamma-, omega-1,2, and omega-5 gliadins in acid polyacrylamide gel electrophoresis. However, the similar mobilities of omega-5 gliadin variants make it difficult to distinguish them among different wheat varieties. In this study, we optimized reverse-phase-ultra-performance liquid chromatography (RP-UPLC) conditions to separate omega-5 gliadins in the reference wheat cultivar Chinese Spring and its nullisomic-tetrasomic lines for chromosome 1B. Five chromatographic peaks corresponded to omega-5 gliadin, and the average relative standard deviation to each peak retention time ranged from 0.31% to 0.93%, indicating that the method is accurate and reproducible for fractionating omega-5 gliadins in gliadin extracts from wheat flour. Using the optimized RP-UPLC method, we analyzed omega-5 gliadins in 24 wheat varieties with the Gli-B1f allele. The result showed that the wheat varieties were sorted into eight groups according to the composition of omega-5 gliadin, indicating that the classification of Gli-B1 alleles based on A-PAGE could not explain the composition of omega-5 gliadin in wheat. We reclassified 73 wheat varieties containing 16 unique Gli-B1 alleles into 31 groups based on the chromatographic patterns of their omega-5 gliadins. Our results provide information on the specific Gli-B1 alleles of wheat varieties belonging to each group and demonstrate the potential for RP-UPLC to facilitate genetic studies of wheat varieties.
|
| 巻・号 |
30(3)
|
| 公開日 |
2025-1-30
|
| DOI |
10.3390/molecules30030609
|
| PII |
molecules30030609
|
| PMID |
39942714
|
| PMC |
PMC11821039
|
| MeSH |
Alleles*
Antigens, Plant
Chromatography, High Pressure Liquid / methods
Chromatography, Reverse-Phase* / methods
Gliadin* / genetics
Polyploidy*
Triticum* / genetics
|
| IF |
3.267
|
| リソース情報 |
| コムギ |
Chinese Spring(CS)
CS-N1AT1B
CS-N1AT1D
CS-N1BT1A
CS-N1BT1D
CS-N1DT1A
CS-N1DT1B |
