論文 - 詳細
| RRC ID | 84317 |
|---|---|
| 著者 | Lee JY, Yang YJ, So J, Kim S, Cho K. |
| タイトル | Allelic Analysis of the Gli-B1 Locus in Hexaploid Wheat Using Reverse-Phase-Ultra-Performance Liquid Chromatography. |
| ジャーナル | Molecules |
| Abstract |
Wheat (Triticum aestivum L.) omega-5 gliadin, a major allergen responsible for wheat-dependent exercise-induced anaphylaxis in humans, is encoded by genes located at the Gli-B1 locus on chromosome 1B, which exhibits genetic polymorphism. Gli-B1 alleles have generally been identified based on the electrophoretic mobilities of the encoded gamma-, omega-1,2, and omega-5 gliadins in acid polyacrylamide gel electrophoresis. However, the similar mobilities of omega-5 gliadin variants make it difficult to distinguish them among different wheat varieties. In this study, we optimized reverse-phase-ultra-performance liquid chromatography (RP-UPLC) conditions to separate omega-5 gliadins in the reference wheat cultivar Chinese Spring and its nullisomic-tetrasomic lines for chromosome 1B. Five chromatographic peaks corresponded to omega-5 gliadin, and the average relative standard deviation to each peak retention time ranged from 0.31% to 0.93%, indicating that the method is accurate and reproducible for fractionating omega-5 gliadins in gliadin extracts from wheat flour. Using the optimized RP-UPLC method, we analyzed omega-5 gliadins in 24 wheat varieties with the Gli-B1f allele. The result showed that the wheat varieties were sorted into eight groups according to the composition of omega-5 gliadin, indicating that the classification of Gli-B1 alleles based on A-PAGE could not explain the composition of omega-5 gliadin in wheat. We reclassified 73 wheat varieties containing 16 unique Gli-B1 alleles into 31 groups based on the chromatographic patterns of their omega-5 gliadins. Our results provide information on the specific Gli-B1 alleles of wheat varieties belonging to each group and demonstrate the potential for RP-UPLC to facilitate genetic studies of wheat varieties. |
| 巻・号 | 30(3) |
| 公開日 | 2025-1-30 |
| DOI | 10.3390/molecules30030609 |
| PII | molecules30030609 |
| PMID | 39942714 |
| PMC | PMC11821039 |
| MeSH | Alleles* Antigens, Plant Chromatography, High Pressure Liquid / methods Chromatography, Reverse-Phase* / methods Gliadin* / genetics Polyploidy* Triticum* / genetics |
| IF | 3.267 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| コムギ | Chinese Spring(CS) CS-N1AT1B CS-N1AT1D CS-N1BT1A CS-N1BT1D CS-N1DT1A CS-N1DT1B |