| RRC ID |
84324
|
| Author |
Sasagawa K, Domon H, Hirayama S, Maekawa T, Isono T, Tabeta K, Terao Y.
|
| Title |
Secretory leucocyte protease inhibitor regulates bone metabolism and inflammation in experimental mouse periodontitis.
|
| Journal |
Commun Biol
|
| Abstract |
Periodontitis is characterized by the activity of neutrophil elastase, a host defense factor that leads to the destruction of the epithelial barrier and bacterial invasion of the periodontal tissue. Secretory leukocyte protease inhibitors (SLPI), predominantly secreted by epithelial cells, diffuse into the mucosal surface and inhibit excessive tissue loss caused by elastase during inflammation. The SLPI level is high in healthy gingiva and low in severe periodontitis. In this study, we hypothesized that intragingival administration of SLPI inhibits periodontal tissue destruction caused by periodontitis. Administration of SLPI significantly reduced neutrophil elastase activity in periodontal tissue and alleviated alveolar bone loss in mice. Real-time PCR analysis revealed that SLPI administration downregulated the transcription of pro-inflammatory cytokines and osteoclast-related factors in the gingival tissue. Furthermore, in vitro treatment of bone marrow macrophages with SLPI resulted in the downregulation of osteoclast differentiation. SLPI treatment of MC3T3-E1 cells promoted osteoblast differentiation and bone formation. These findings suggest that SLPI protects against periodontal tissue damage by suppressing inflammation and bone resorption and promoting bone regeneration.
|
| Volume |
8(1)
|
| Pages |
760
|
| Published |
2025-5-16
|
| DOI |
10.1038/s42003-025-08197-3
|
| PII |
10.1038/s42003-025-08197-3
|
| PMID |
40379839
|
| PMC |
PMC12084318
|
| MeSH |
Alveolar Bone Loss / metabolism
Animals
Bone and Bones* / drug effects
Bone and Bones* / metabolism
Cell Differentiation / drug effects
Disease Models, Animal
Inflammation / metabolism
Leukocyte Elastase / metabolism
Male
Mice
Mice, Inbred C57BL
Osteoclasts / drug effects
Osteoclasts / metabolism
Osteogenesis / drug effects
Periodontitis* / drug therapy
Periodontitis* / metabolism
Periodontitis* / pathology
Secretory Leukocyte Peptidase Inhibitor* / metabolism
Secretory Leukocyte Peptidase Inhibitor* / pharmacology
|
| IF |
4.165
|
| Resource |
| Human and Animal Cells |
MC3T3-E1(RCB1126) |
