Reference - Detail
| RRC ID | 84330 |
|---|---|
| Author | Tabara M, Harada M, Kuriyama K, Sakamoto T, Takeda A, Fukuhara T, Tabunoki H. |
| Title | Biochemical characterization of Bombyx mori Dicer-2 that dices double-stranded RNAs into 20-nt small RNA. |
| Journal | Arch Insect Biochem Physiol |
| Abstract |
We detected enzymatic activity that generates 20-nucleotide (nt) RNA from double-stranded RNAs (dsRNAs) in crude extracts prepared from various silkworm (Bombyx mori) organs. The result using knocked-down cultured cells indicated that this dicing activity originated from B. mori Dicer-2 (BmDcr2). Biochemical analyses revealed that BmDcr2 preferentially cleaves 5'-phosphorylated dsRNAs at the 20-nt site-counted from the 5'-phosphorylated end-and required ATP and magnesium ions for the dicing reaction. This is the first report of the biochemical characterization of Dicer-2 in lepidopteran insects. This enzymatic property of BmDcr2 in vitro is consistent with the in vivo small interfering RNA profile in virus-infected silkworm cells. |
| Volume | 116(1) |
| Pages | e22118 |
| Published | 2024-5-1 |
| DOI | 10.1002/arch.22118 |
| PMID | 38713637 |
| MeSH | Animals Bombyx* / genetics Bombyx* / metabolism Insect Proteins / genetics Insect Proteins / metabolism Larva / genetics Larva / growth & development Larva / metabolism Magnesium / metabolism RNA, Double-Stranded* / metabolism RNA, Small Interfering / metabolism Ribonuclease III* / genetics Ribonuclease III* / metabolism |
| IF | 1.536 |
| Resource | |
| Silkworms | N4 |