論文 - 詳細
| RRC ID | 84337 |
|---|---|
| 著者 | Nakata M, Ueno M, Kikuchi Y, Iwami M, Takayanagi-Kiya S, Kiya T. |
| タイトル | CRISPR/Cas9- and Single-Stranded ODN-Mediated Knock-In in Silkworm Bombyx mori. |
| ジャーナル | Zoolog Sci |
| Abstract |
Although genome editing techniques have made significant progress, introducing exogenous genes into the genome through knock-in remains a challenge in many organisms. In silkworm Bombyx mori, TALEN-mediated knock-in methods have been established. However, difficulties in construction and limitations of the target sequence have hindered the application of these methods. In the present study, we verified several CRISPR/Cas9-mediated knock-in methods to expand the application of gene knock-in techniques and found that the short single-stranded oligodeoxynucleotide (ssODN)-mediated method is the most effective in silkworms. Using ssODN-mediated methods, we established knock-in silkworm strains that harbor an attP sequence, a 50 bp phiC31 integrase recognition site, at either the BmHr38 (Hormone receptor 38) or Bmdsx (doublesex) locus. Additionally, we found that the long ssODN (lsODN)-mediated method successfully introduced the GAL4 gene at the doublesex locus in embryos. The present study provides valuable information on CRISPR/Cas9-mediated knock-in methods in silkworms, expanding the utility of genome editing techniques in insects and paving the way for analyzing gene and genome function in silkworms. |
| 巻・号 | 41(6) |
| ページ | 540-547 |
| 公開日 | 2024-12-1 |
| DOI | 10.2108/zs240019 |
| PMID | 39636137 |
| MeSH | Animals Bombyx* / genetics CRISPR-Cas Systems* Gene Editing Gene Knock-In Techniques* Oligodeoxyribonucleotides / genetics |
| IF | 0.843 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| カイコ | N4 and w1-pnd |