Reference - Detail
| RRC ID | 84342 |
|---|---|
| Author | De Padua JC, Tanaka T, Ueno K, Dela Cruz TEE, Ishihara A. |
| Title | Isolation of 2,2'-azoxybisbenzyl alcohol from Agaricus subrutilescens and its inhibitory activity against bacterial biofilm formation. |
| Journal | Biosci Biotechnol Biochem |
| Abstract |
Virulence pathways in pathogenic bacteria are regulated by quorum sensing mechanisms, particularly biofilm formation through autoinducer (AI) production and sensing. In this study, the culture filtrate extracted from an edible mushroom, Agaricus subrutilescens, was fractionated to isolate a compound that inhibits biofilm formation. Four gram-negative bacteria (Klebsiella pneumoniae, Escherichia coli, Proteus mirabilis, and Enterobacter cloacae) and two gram-positive bacteria (Enterococcus faecalis and Staphylococcus aureus) were used for the bioassay. The bioassay-guided chromatographic separations of the culture filtrate extract resulted in the isolation of the compound. Further, spectroscopic analyses revealed the identity of the compound as 2,2'-azoxybisbenzyl alcohol (ABA). The minimum inhibitory and sub-inhibitory concentrations of the compound were also determined. Azoxybisbenzyl alcohol was significantly effective in inhibiting biofilm formation in all tested bacteria, with half-maximal inhibitory concentrations of 3-11 µg/mL. Additionally, the bioactivity of ABA was confirmed through the bioassays for the inhibition of exopolysaccharide matrixes and AI activities. |
| Volume | 88(9) |
| Pages | 983-991 |
| Published | 2024-8-26 |
| DOI | 10.1093/bbb/zbae089 |
| PII | 7699867 |
| PMID | 38925646 |
| MeSH | Agaricus* / chemistry Anti-Bacterial Agents* / chemistry Anti-Bacterial Agents* / isolation & purification Anti-Bacterial Agents* / pharmacology Biofilms* / drug effects Microbial Sensitivity Tests* Quorum Sensing / drug effects |
| IF | 1.516 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Fungus/Mushroom | TUFC 100542 |