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RRC ID 84363
著者 Fujii T, Hino M, Fujimoto T, Kakino K, Kaneko Y, Abe H, Lee JM, Kusakabe T, Shimada T.
タイトル Peroxiredoxin 6 is essential for the posttranslational activation of xanthine dehydrogenase in the uric acid synthesis of Bombyx mori.
ジャーナル Insect Biochem Mol Biol
Abstract We identified a novel mutant of Bombyx mori, designated as male-absent oily (genetic symbol: om). The larval integument of this mutant is translucent due to a lack of uric acid in the integument. This mutation is Z-linked, and as mutant females are infertile, it is impossible to obtain om homozygous males. Using positional cloning combined with RNA-seq analysis, we identified a 1-bp deletion in the B. mori peroxiredoxin 6 (BmPrx6) gene. CRISPR/Cas9 knockout of BmPrx6 resulted in a translucent larval integument, indicating BmPrx6 as the causative gene for the om locus. Xanthine dehydrogenase (XDH)/xanthine oxidase (XO) is a key enzyme for uric acid synthesis. Injection of bovine XO into om mutants rescued the translucent phenotype, indicating that om is a mutant with defective XDH activity. To investigate XDH in B. mori, we generated a FLAG-tagged XDH gene using the CRISPR/Cas9 knock-in approach. Western blot analysis of XDH in om mutants revealed that BmPrx6 is crucial for the posttranslational activation of XDH. The role of BmPrx6 in regulating XDH activity is discussed.
巻・号 178
ページ 104264
公開日 2025-3-1
DOI 10.1016/j.ibmb.2025.104264
PII S0965-1748(25)00008-6
PMID 39889859
MeSH Animals Bombyx* / enzymology Bombyx* / genetics Bombyx* / growth & development Bombyx* / metabolism CRISPR-Cas Systems Female Insect Proteins* / genetics Insect Proteins* / metabolism Larva / genetics Larva / growth & development Larva / metabolism Male Peroxiredoxins* / genetics Peroxiredoxins* / metabolism Protein Processing, Post-Translational Uric Acid* / metabolism Xanthine Dehydrogenase* / genetics Xanthine Dehydrogenase* / metabolism
IF 3.827
リソース情報
カイコ F02 k151 WZE n41 p50 p50ma p55