Reference - Detail
| RRC ID | 84389 |
|---|---|
| Author | Niimi H, Onishi T, Nomura S, Kumazaki A, Masaki Y, Odashima H, Osawa Y, Hattori M. |
| Title | Assessment of Nucleic Acid Quality in Unstained Cytology Specimens for Cancer Genomic Testing. |
| Journal | Acta Cytol |
| Abstract |
INTRODUCTION:The use of cytological specimens in cancer genome medicine has garnered considerable attention, but the long-term quality of nucleic acids from unstained specimens remains unclear. This study aimed to evaluate the quality of nucleic acids extracted from unstained specimens fixed with 95% ethanol or spray fixation over varying durations. METHODS:Two lung cancer cell lines were prepared using the auto-smear method and fixed with 95% ethanol, and spray-fixed specimens were stored for 30 min, 1 day, 3 days, 1 week, 2 weeks, 1 month, 3 months, and 6 months. DNA was extracted using a DNA extraction kit, and quality was assessed using agarose gel electrophoresis and PCR. RESULTS:Nucleic acids extracted from unstained specimens showed no fragmentation after 6 months of fixation and were amplifiable by PCR, regardless of the fixation method. CONCLUSION:Nucleic acids extracted from unstained specimens preserved high quality over 6 months, suggesting that such specimens are suitable for genetic testing. This finding has significant implications for the long-term storage and clinical application of cytological specimens in cancer genome medicine. |
| Pages | 1-8 |
| Published | 2025-3-24 |
| DOI | 10.1159/000545423 |
| PII | 000545423 |
| PMID | 40127635 |
| MeSH | Cell Line, Tumor Cytodiagnosis* / methods Electrophoresis, Agar Gel Genetic Testing* / methods Humans Lung Neoplasms* / genetics Lung Neoplasms* / pathology Polymerase Chain Reaction Specimen Handling / methods Time Factors Tissue Fixation / methods |
| IF | 1.226 |
| Resource | |
| Human and Animal Cells | A549 |