Reference - Detail
| RRC ID | 84404 |
|---|---|
| Author | Iikawa H, Nishina A, Morita M, Atsuta Y, Hayashi Y, Saito D. |
| Title | Labeling and sorting of avian primordial germ cells utilizing Lycopersicon Esculentum lectin. |
| Journal | Dev Growth Differ |
| Abstract |
Avian species are essential resources for human society, with their preservation and utilization heavily dependent on primordial germ cells (PGCs). However, efficient methods for isolating live PGCs from embryos remain elusive in avian species beyond chickens, and even in chickens, existing techniques have shown limited efficiency. In this study, we present a rapid, simple, and cost-effective method for labeling and sorting circulating-stage PGCs across various avian species, including Carinatae and Ratitae, using Lycopersicon Esculentum (Tomato) lectin (LEL). Notably, this method demonstrates high sorting efficiency by identifying a wide range of PGC subtypes while preserving the proliferative and migratory potential of chicken PGCs. This approach is anticipated to significantly contribute to the conservation, research, and agricultural industries related to avian species globally. |
| Volume | 66(9) |
| Pages | 452-461 |
| Published | 2024-12-1 |
| DOI | 10.1111/dgd.12948 |
| PMID | 39520225 |
| PMC | PMC11659090 |
| MeSH | Animals Birds / embryology Birds / metabolism Chickens Germ Cells* / cytology Germ Cells* / metabolism Plant Lectins / metabolism |
| IF | 1.723 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Chicken / Quail | BL‐E strain of brawn leghorn WE strain |