Reference - Detail
| RRC ID | 84513 |
|---|---|
| Author | Wang C, Le MN-T, Kawada-Matsuo M, Hisatsune J, Sugawara Y, Arai C, Nakanishi J, Takeda K, Shiba H, Sugai M, Komatsuzawa H. |
| Title | Ursoricin, a bacteriocin of Streptococcus ursoris, has potent activity against methicillin-resistant Staphylococcus aureus and vancomycin-resistant enterococci. |
| Journal | Appl Environ Microbiol |
| Abstract |
UNLABELLED:The emergence of drug-resistant bacteria, particularly methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant enterococci (VRE), has increased the need to discover novel antimicrobial agents that are effective against these species. Here, we describe the identification and purification of the mutacin BHT-B-like gene locus and bacteriocin peptide from Streptococcus ursoris, which is closely related to Streptococcus ratti; hence, we named this bacteriocin ursoricin. Ursoricin is a cationic, chromosome-encoded peptide that has potent antimicrobial effects against Gram-positive pathogens, including MRSA and VRE, with minimum inhibitory concentrations in the micromolar range. Ursoricin also inhibits the biofilm formation of high biofilm-forming S. aureus. Antibacterial activity was retained after treatment at 100°C for 60 min at a pH range of 3-9 and was partially reduced by treatment with proteinase K for 2 h (63% residual activity). The potent anti-MRSA, anti-VRE, and antibiofilm effects of ursoricin suggest that it is a possible candidate for the treatment of MRSA, VRE, and biofilm-associated infections. IMPORTANCE:The emergence of multidrug-resistant bacteria worldwide has posed a significant public health threat and economic burdens that make the identification and development of novel antimicrobial agents urgent. Bacteriocins are promising new agents that exhibit antibacterial activity against a wide range of human pathogens. In this study, we report that the bacteriocin produced by Streptococcus ursoris showed good antibacterial activity against a wide range of Staphylococcus aureus and enterococcus strains, particularly methicillin-resistant Staphylococcus aureus, vancomycin-resistant enterococci, and high biofilm-forming S. aureus. Interestingly, this bacteriocin had a stronger effect on S. aureus than on Staphylococcus epidermidis, which is a major commensal bacterium in human skin; this result is important when considering the disturbance of bacterial flora, especially on the skin, mediated by the application of antibacterial agents. |
| Volume | 90(6) |
| Pages | e0016224 |
| Published | 2024-6-18 |
| DOI | 10.1128/aem.00162-24 |
| PMID | 38775468 |
| PMC | PMC11218630 |
| MeSH | Anti-Bacterial Agents* / pharmacology Bacteriocins* / genetics Bacteriocins* / pharmacology Biofilms* / drug effects Methicillin-Resistant Staphylococcus aureus* / drug effects Microbial Sensitivity Tests* Streptococcus* / drug effects Vancomycin-Resistant Enterococci* / drug effects |
| Resource | |
| General Microbes | JCM31912 JCM2416 JCM2420 JCM2424 JCM20595 JCM1464 |