論文 - 詳細
| RRC ID | 84517 |
|---|---|
| 著者 | Matsuzaki C, Takagi H, Saiga S, Kinoshita Y, Yamaguchi M, Higashimura Y, Yamamoto K, Yamaguchi M. |
| タイトル | Prebiotic effect of galacto-N-biose on the intestinal lactic acid bacteria as enhancer of acetate production and hypothetical colonization. |
| ジャーナル | Appl Environ Microbiol |
| Abstract |
UNLABELLED:Galacto-N-biose (GNB) is an important core structure of glycan of mucin glycoproteins in the gastrointestinal (GI) mucosa. Because certain beneficial bacteria inhabiting the GI tract, such as bifidobacteria and lactic acid bacteria, harbor highly specialized GNB metabolic capabilities, GNB is considered a promising prebiotic for nourishing and manipulating beneficial bacteria in the GI tract. However, the precise interactions between GNB and beneficial bacteria and their accompanying health-promoting effects remain elusive. First, we evaluated the proliferative tendency of beneficial bacteria and their production of beneficial metabolites using gut bacterial strains. By comparing the use of GNB, glucose, and inulin as carbon sources, we found that GNB enhanced acetate production in Lacticaseibacillus casei, Lacticaseibacillus rhamnosus, Lactobacillus gasseri, and Lactobacillus johnsonii. The ability of GNB to promote acetate production was also confirmed by RNA-seq analysis, which indicated the upregulation of gene clusters that catalyze the deacetylation of N-acetylgalactosamine-6P and biosynthesize acetyl-CoA from pyruvate, both of which result in acetate production. To explore the in vivo effect of GNB in promoting acetate production, antibiotic-treated BALB/cA mice were administered with GNB with L. rhamnosus, resulting in a fecal acetate content that was 2.7-fold higher than that in mice administered with only L. rhamnosus. Moreover, 2 days after the last administration, a 3.7-fold higher amount of L. rhamnosus was detected in feces administered with GNB with L. rhamnosus than in feces administered with only L. rhamnosus. These findings strongly suggest the prebiotic potential of GNB in enhancing L. rhamnosus colonization and converting L. rhamnosus into higher acetate producers in the GI tract. IMPORTANCE:Specific members of lactic acid bacteria, which are commonly used as probiotics, possess therapeutic properties that are vital for human health enhancement by producing immunomodulatory metabolites such as exopolysaccharides, short-chain fatty acids, and bacteriocins. The long residence time of probiotic lactic acid bacteria in the GI tract prolongs their beneficial health effects. Moreover, the colonization property is also desirable for the application of probiotics in mucosal vaccination to provoke a local immune response. In this study, we found that GNB could enhance the beneficial properties of intestinal lactic acid bacteria that inhabit the human GI tract, stimulating acetate production and promoting intestinal colonization. Our findings provide a rationale for the addition of GNB to lactic acid bacteria-based functional foods. This has also led to the development of therapeutics supported by more rational prebiotic and probiotic selection, leading to an improved healthy lifestyle for humans. |
| 巻・号 | 90(3) |
| ページ | e0144523 |
| 公開日 | 2024-3-20 |
| DOI | 10.1128/aem.01445-23 |
| PMID | 38411084 |
| PMC | PMC10952502 |
| MeSH | Acetates Animals Bacteria Disaccharidases Humans Lactobacillales* / genetics Mice Prebiotics Probiotics* / metabolism |
| リソース情報 | |
| 一般微生物 | JCM1136 JCM5827 JCM15633 JCM5824 JCM9498 JCM13265 JCM9496 JCM5828 JCM5826 JCM13471 JCM5825 JCM9497 JCM13406 JCM30893 JCM1275 JCM1195 JCM7096 JCM1194 JCM1255 JCM1192 JCM1222 JCM1217 JCM10602 JCM1205 JCM10188 JCM31500 JCM14655 JCM1395 JCM15631 JCM6421 JCM1298 JCM1397 JCM6124 JCM17834 JCM5805 JCM5803 JCM1185 JCM2012 JCM1131 JCM1112 JCM1173 JCM1149 JCM1231 JCM1134 JCM8130 |