論文 - 詳細
| RRC ID | 84519 |
|---|---|
| 著者 | Kaweewan I, Mukai K, Rukthanapitak P, Nakagawa H, Hosaka T, Kodani S. |
| タイトル | Heterologous biosynthesis of myxobacterial lanthipeptides melittapeptins. |
| ジャーナル | Appl Microbiol Biotechnol |
| Abstract |
The myxobacteria are an attractive bioresource for bioactive compounds since the large size genome contains many biosynthetic gene clusters of secondary metabolites. The genome of the myxobacterium Melittangium boletus contains three biosynthetic gene clusters for lanthipeptide production. One of the gene clusters includes genes coding lanthipeptide precursor (melA), class II lanthipeptide synthetase (melM), and transporter (melT). The amino acid sequence of melA indicated similarity with that of known lanthipeptides mersacidin and lichenicidin A1 by the alignment. To perform heterologous production of new lanthipeptides, the expression vector containing the essential genes (melA and melM) was constructed by utilizing codon-optimized synthetic genes. The co-expression of two genes in the host bacterial cells of Escherichia coli BL21 (DE3) afforded new lanthipeptides named melittapeptins A-C. The structures of melittapeptins A-C including lanthionine/methyllanthionine bridge pattern were proposed based on protease digestion and MS/MS experiments. The native strain of M. boletus did not produce melittapeptins A-C, so heterologous production using the biosynthetic gene cluster was effective in obtaining the lanthipeptides. Melittapeptins A-C showed specific and potent antibacterial activity to the Gram-positive bacterium Micrococcus luteus. To the best of our knowledge, this is the first report of antibacterial lanthipeptides derived from myxobacterial origin. KEY POINTS: • New lanthipeptides melittapeptins were heterologously produced in Escherichia coli. • Melittapeptins showed specific antibacterial activity against Micrococcus luteus. • Melittapeptins were the first antibacterial lanthipeptides of myxobacterial origin. |
| 巻・号 | 108(1) |
| ページ | 122 |
| 公開日 | 2024-12-1 |
| DOI | 10.1007/s00253-023-12834-4 |
| PII | 10.1007/s00253-023-12834-4 |
| PMID | 38229328 |
| MeSH | Amino Acid Sequence Anti-Bacterial Agents / metabolism Bacteriocins* / genetics Bacteriocins* / pharmacology Escherichia coli / genetics Escherichia coli / metabolism Myxococcales* / genetics Myxococcales* / metabolism Tandem Mass Spectrometry |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM5802 JCM1310 |