| RRC ID |
84543
|
| Author |
Kojima K, Wakabayashi Y, Nishijima S, Sakata J, Sekiya S, Iwamoto S, Tanaka K.
|
| Title |
Characterisation of glucose-induced protein fragments among the order Enterobacterales using matrix-assisted laser desorption ionization-time of flight mass spectrometry.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
To characterise the glucose-induced protein fragments by MALDI-TOF MS analysis, we compared data for samples from Escherichia coli cultured in media with or without glucose. Characteristic peaks were observed in the presence of glucose, and MS/MS revealed Asr-specific fragments. The amino acid sequences of the fragments suggested sequence-specific proteolysis. Blast-analysis revealed that numerous Enterobacterales harbored genes encoding Asr as well as E. coli. Here, we analysed 32 strains from 20 genera and 25 species of seven Enterobacterales families. We did not detect changes in the mass spectra of four strains of Morganellaceae lacking asr, whereas peaks of Asr-specific fragments were detected in the other 28 strains. We therefore concluded that the induction of Asr production in the presence of glucose is common among the Enterobacterales, except for certain Morganellaceae species. In members of family Budviciaceae, unfragmented Asr was detected. Molecular genetic information suggested that the amino acid sequences of Asr homologs are diverse, with fragments varying in number and size, indicating that Asr may serve as a discriminative biomarker for identifying Enterobacterales species.
|
| Volume |
732
|
| Pages |
150407
|
| Published |
2024-11-5
|
| DOI |
10.1016/j.bbrc.2024.150407
|
| PII |
S0006-291X(24)00943-4
|
| PMID |
39033555
|
| MeSH |
Amino Acid Sequence
Bacterial Proteins / chemistry
Bacterial Proteins / genetics
Enterobacteriaceae* / genetics
Enterobacteriaceae* / metabolism
Glucose* / metabolism
Molecular Sequence Data
Peptide Fragments / chemistry
Peptide Fragments / genetics
Peptide Fragments / metabolism
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization* / methods
|
| Resource |
| General Microbes |
JCM1233
JCM15110
JCM30077
JCM1673
JCM3902
JCM5902
JCM5900
JCM24417 |
