| RRC ID |
84594
|
| 著者 |
Saroj P, P M, Narasimhulu K.
|
| タイトル |
Enhanced reducing sugar production by blending hydrolytic enzymes from Aspergillus fumigatus to improve sugarcane bagasse hydrolysis.
|
| ジャーナル |
Environ Sci Pollut Res Int
|
| Abstract |
Biomass pretreatment for the production of second-generation (2G) ethanol and biochemical products is a challenging process. The present study investigated the synergistic efficiency of purified carboxymethyl cellulase (CMCase), β-glucosidase, and xylanase from Aspergillus fumigatus JCM 10253 in the hydrolysis of alkaline-pretreated sugarcane bagasse (SCB). The saccharification of pretreated SCB was optimised using a combination of CMCase and β-glucosidase (C + β; 1:1) and addition of xylanase (C + β + xyl; 1:1:1). Independent and dependent variables influencing enzymatic hydrolysis were investigated using response surface methodology (RSM). Hydrolysis using purified CMCase and β-glucosidase achieved yields of 18.72 mg/mL glucose and 6.98 mg/mL xylose. Incorporation of xylanase in saccharification increased the titres of glucose (22.83 mg/mL) and xylose (9.54 mg/mL). Furthermore, characterisation of SCB biomass by scanning electron microscopy, X-ray diffraction, and Fourier transform infrared spectroscopy respectively confirmed efficient structural disintegration and revealed the degree of crystallinity and spectral characteristics. Therefore, depolymerisation of lignin to produce high-value chemicals is essential for sustainable and competitive biorefinery development.
|
| 巻・号 |
31(35)
|
| ページ |
48085-48102
|
| 公開日 |
2024-7-1
|
| DOI |
10.1007/s11356-024-34246-1
|
| PII |
10.1007/s11356-024-34246-1
|
| PMID |
39017871
|
| MeSH |
Aspergillus fumigatus* / enzymology
Biomass*
Cellulase / metabolism
Cellulose*
Hydrolysis
Saccharum*
Sugars / metabolism
Xylose / metabolism
beta-Glucosidase / metabolism
|
| リソース情報 |
| 一般微生物 |
JCM10253 |
