| RRC ID |
84596
|
| 著者 |
Iketani A, Takano M, Kasakura K, Iwatsuki M, Tsuji A, Matsuda K, Minegishi R, Hosono A, Nakanishi Y, Takahashi K.
|
| タイトル |
CCAAT/enhancer-binding protein α-dependent regulation of granule formation in mast cells by intestinal bacteria.
|
| ジャーナル |
Eur J Immunol
|
| Abstract |
The antiallergic effects of gut microbiota have been attracting attention in recent years, but the underlying cellular and molecular mechanisms have not yet been fully understood. In this study, we aimed to investigate these mechanisms specifically focusing on mast cells. Mast cells retain intracellular granules containing various inflammatory mediators such as histamine, which are released outside the cells upon IgE and allergen stimulation. We previously reported that increased expression of the transcription factor, CCAAT/enhancer-binding protein α (C/EBPα), suppresses granule formation in mast cells and that Lacticaseibacillus casei JCM1134T (LC) upregulates C/EBPα levels. Here, granule formation in mouse bone marrow-derived mast cells was suppressed in a MyD88-dependent manner after LC treatment due to C/EBPα-dependent downregulation of the genes encoding serglycin (SRGN) and mast cell protease 4 (Mcpt4). Furthermore, C/EBPα expression was regulated by DNA methylation in the 5' region far upstream of the transcription start site. LC suppressed DNA methylation of specific CpG motifs in the 5' region of the C/EBPα gene. These results conclude that specific gut microbial components, such as those from LC, suppress granule formation in mast cells by inhibiting SRGN and Mcpt4 expression via reduced C/EBPα gene methylation.
|
| 巻・号 |
54(10)
|
| ページ |
e2451094
|
| 公開日 |
2024-10-1
|
| DOI |
10.1002/eji.202451094
|
| PMID |
38980255
|
| MeSH |
Animals
CCAAT-Enhancer-Binding Protein-alpha / genetics
CCAAT-Enhancer-Binding Protein-alpha / metabolism
Cell Degranulation / immunology
DNA Methylation
Gastrointestinal Microbiome* / immunology
Gene Expression Regulation / immunology
Lacticaseibacillus casei / immunology
Mast Cells* / immunology
Mast Cells* / metabolism
Mice
Mice, Inbred C57BL
Mice, Knockout
Myeloid Differentiation Factor 88 / genetics
Myeloid Differentiation Factor 88 / metabolism
|
| リソース情報 |
| 一般微生物 |
JCM1134 |
