| RRC ID |
84599
|
| Author |
Nithimethachoke T, Boonmak C, Morikawa M.
|
| Title |
A novel alkane monooxygenase evolved from a broken piece of ribonucleotide reductase in Geobacillus kaustophilus HTA426 isolated from Mariana Trench.
|
| Journal |
Extremophiles
|
| Abstract |
We have accidentally found that a thermophilic Geobacillus kaustophilus HTA426 is capable of degrading alkanes although it has no alkane oxygenating enzyme genes. Our experimental results revealed that a putative ribonucleotide reductase small subunit GkR2loxI (GK2771) gene encodes a novel heterodinuclear Mn-Fe alkane monooxygenase/hydroxylase. GkR2loxI protein can perform two-electron oxidations similar to homonuclear diiron bacterial multicomponent soluble methane monooxygenases. This finding not only answers a long-standing question about the substrate of the R2lox protein clade, but also expands our understanding of the vast diversity and new evolutionary lineage of the bacterial alkane monooxygenase/hydroxylase family.
|
| Volume |
28(1)
|
| Pages |
18
|
| Published |
2024-2-14
|
| DOI |
10.1007/s00792-024-01332-8
|
| PII |
10.1007/s00792-024-01332-8
|
| PMID |
38353731
|
| PMC |
PMC10867098
|
| MeSH |
Alkanes
Geobacillus* / genetics
Mixed Function Oxygenases / genetics
Ribonucleotide Reductases* / genetics
|
| Resource |
| General Microbes |
JCM12893 |
