| RRC ID |
84639
|
| 著者 |
Nirwantono R, Laksmi FA, Nuryana I, Firdausa S, Herawan D, Giyandini R, Hidayat AA.
|
| タイトル |
Exploring an l-arabinose isomerase from cryophile bacteria Arthrobacter psychrolactophilus B7 for d-tagatose production.
|
| ジャーナル |
Int J Biol Macromol
|
| Abstract |
A novel l-arabinose isomerase (L-AI) from Arthrobacter psychrolactophilus (Ap L-AI) was successfully cloned and characterized. The enzyme catalyzes the isomerization of d-galactose into a rare sugar d-tagatose. The recombinant Ap L-AI had an approximate molecular weight of about 258 kDa, suggesting it was an aggregate of five 58 kDa monomers and became the first record as a homo-pentamer L-AI. The catalytic efficiency (kcat/Km) and Km for d-galactose were 0.32 mM-1 min-1 and 51.43 mM, respectively, while for l-arabinose, were 0.64 mM-1 min-1 and 23.41 mM, respectively. It had the highest activity at pH 7.0-7.5 and 60 °C in the presence of 0.250 mM Mn2+. Ap L-AI was discovered to be an outstanding thermostable enzyme that only lost its half-life value at 60 °C for >1000 min. These findings suggest that l-arabinose isomerase from Arthrobacter psychrolactophilus is a promising candidate for d-tagatose mass-production due to its industrially competitive temperature.
|
| 巻・号 |
254(Pt 2)
|
| ページ |
127781
|
| 公開日 |
2024-1-1
|
| DOI |
10.1016/j.ijbiomac.2023.127781
|
| PII |
S0141-8130(23)04680-9
|
| PMID |
37923040
|
| MeSH |
Aldose-Ketose Isomerases* / chemistry
Arthrobacter*
Cloning, Molecular
Galactose / chemistry
Hexoses / chemistry
Hydrogen-Ion Concentration
Recombinant Proteins / genetics
|
| リソース情報 |
| 一般微生物 |
JCM12399 |
