| RRC ID |
84652
|
| 著者 |
Yamanaka R, Usui M, Kobayashi K, Onizuka S, Kasai S, Sano K, Hironaka S, Yamasaki R, Yoshii S, Sato T, Fujii W, Iwasaki M, Ariyoshi W, Nakashima K, Nishihara T.
|
| タイトル |
Evaluation of a Novel Immunochromatographic Device for Detecting Porphyromonas gingivalis in Patients with Periodontal Disease.
|
| ジャーナル |
Int J Mol Sci
|
| Abstract |
Porphyromonas gingivalis is the most pathogenic periodontal bacterium in the world. Recently, P. gingivalis has been considered responsible for dysbiosis during the development of periodontitis. This study aimed to evaluate a novel immunochromatographic device using monoclonal antibodies against P. gingivalis in subgingival plaques. A total of 72 patients with chronic periodontitis and 53 periodontally healthy volunteers underwent clinical and microbiological examinations. Subgingival plaque samples were analyzed for the presence of P. gingivalis and compared using real-time polymerase chain reaction (PCR). In the periodontitis group, a significant positive correlation was observed between the test device scores and the real-time PCR results. The specificity, positive predictive value, negative predictive value, and accuracy of the test device for P. gingivalis, as determined by real-time PCR, were 98%, 94%, 89%, and 90%, respectively. There were significant differences in bacterial counts by real-time PCR among the groups with different ranges of device scores. Additionally, there was a significant positive correlation between the device scores for P. gingivalis and periodontal parameters. These results suggest that this novel immunochromatographic device can be effectively used for rapid detection and semi-quantification of P. gingivalis in subgingival plaques.
|
| 巻・号 |
25(15)
|
| 公開日 |
2024-7-26
|
| DOI |
10.3390/ijms25158187
|
| PII |
ijms25158187
|
| PMID |
39125757
|
| PMC |
PMC11311996
|
| MeSH |
Adult
Chromatography, Affinity* / instrumentation
Chromatography, Affinity* / methods
Chronic Periodontitis / diagnosis
Chronic Periodontitis / microbiology
Dental Plaque / microbiology
Female
Humans
Male
Middle Aged
Periodontal Diseases / diagnosis
Periodontal Diseases / microbiology
Porphyromonas gingivalis* / immunology
Porphyromonas gingivalis* / isolation & purification
Real-Time Polymerase Chain Reaction / methods
Sensitivity and Specificity
|
| リソース情報 |
| 一般微生物 |
JCM12257 |
