| RRC ID |
84685
|
| Author |
Peng C, Zhang X, Li J, Yang M, Ma S, Fan H, Dai L, Cheng L.
|
| Title |
Oleispirillum naphthae gen. nov., sp. nov., a bacterium isolated from oil sludge, and proposal of Oleispirillaceae fam. nov.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
A microaerophilic, Gram-negative, motile, and spiral-shaped bacterium, designated Y-M2T, was isolated from oil sludge of Shengli oil field. The optimal growth condition of strain Y-M2T was at 25 °C, pH 7.0, and in the absence of NaCl. The major polar lipid was phosphatidylethanolamine. The main cellular fatty acid was iso-C17 : 0 3-OH. It contained Q-9 and Q-10 as the predominant quinones. The DNA G+C content was 68.1 mol%. Strain Y-M2T showed the highest 16S rRNA gene sequence similarity to Telmatospirillum siberiense 26-4bT (91.1 %). Phylogenetic analyses based on 16S rRNA gene and genomes showed that strain Y-M2T formed a distinct cluster in the order Rhodospirillales. Genomic analysis showed that Y-M2T possesses a complete nitrogen-fixation cluster which is phylogenetically close to that of methanogene. The nif cluster, encompassing the nitrogenase genes, was found in every N2-fixing strain within the order Rhodospirillales. Phylogeny, phenotype, chemotaxonomy, and genomic results demonstrated that strain Y-M2T represents a novel species of a novel genus in a novel family Oleispirillaceae fam. nov. in the order Rhodospirillales, for which the name Oleispirillum naphthae gen. nov., sp. nov. was proposed. The type strain is Y-M2T (=CCAM 827T=JCM 34765T).
|
| Volume |
74(3)
|
| Published |
2024-3-1
|
| DOI |
10.1099/ijsem.0.006292
|
| PMID |
38512751
|
| PMC |
PMC10963914
|
| MeSH |
Bacterial Typing Techniques
Base Composition
DNA, Bacterial / genetics
Fatty Acids* / chemistry
Phospholipids* / chemistry
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Sewage / microbiology
|
| Resource |
| General Microbes |
JCM34765 |
