Reference - Detail
| RRC ID | 84790 |
|---|---|
| Author | Johnson MD, Sakai HD, Paul B, Nunoura T, Dalvi S, Mudaliyar M, Shepherd DC, Shimizu M, Udupa S, Ohkuma M, Kurosawa N, Ghosal D. |
| Title | Large attachment organelle mediates interaction between Nanobdellota archaeon YN1 and its host. |
| Journal | ISME J |
| Abstract |
DPANN archaea are an enigmatic superphylum that are difficult to isolate and culture in the laboratory due to their specific culture conditions and apparent ectosymbiotic lifestyle. Here, we successfully isolated and cultivated a coculture system of a novel Nanobdellota archaeon YN1 and its host Sulfurisphaera ohwakuensis YN1HA. We characterized the coculture system by complementary methods, including metagenomics and metabolic pathway analysis, fluorescence microscopy, and high-resolution electron cryo-tomography (cryoET). We show that YN1 is deficient in essential metabolic processes and requires host resources to proliferate. CryoET imaging revealed an enormous attachment organelle present in the YN1 envelope that forms a direct interaction with the host cytoplasm, bridging the two cells. Together, our results unravel the molecular and structural basis of ectosymbiotic relationship between YN1 and YN1HA. This research broadens our understanding of DPANN biology and the versatile nature of their ectosymbiotic relationships. |
| Volume | 18(1) |
| Published | 2024-1-8 |
| DOI | 10.1093/ismejo/wrae154 |
| PII | 7729375 |
| PMID | 39113594 |
| PMC | PMC11420986 |
| MeSH | Coculture Techniques Cryoelectron Microscopy Desulfurococcaceae / genetics Desulfurococcaceae / metabolism Electron Microscope Tomography Metagenomics Nanoarchaeota / genetics Nanoarchaeota / metabolism Organelles* / metabolism Organelles* / ultrastructure Symbiosis* |
| Altmetric score |
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 36 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| General Microbes | JCM15769 JCM16833 JCM8930 |