RRC ID 84890
著者 Nagakubo T, Nishiyama T, Yamamoto T, Nomura N, Toyofuku M.
タイトル Contractile injection systems facilitate sporogenic differentiation of Streptomyces davawensis through the action of a phage tapemeasure protein-related effector.
ジャーナル Nat Commun
Abstract Contractile injection systems (CISs) are prokaryotic phage tail-like nanostructures loading effector proteins that mediate various biological processes. Although CIS functions have been diversified through evolution and hold the great potential as protein delivery systems, the functional characterisation of CISs and their effectors is currently limited to a few CIS lineages. Here, we show that the CISs of Streptomyces davawensis belong to a unique group of bacterial CISs distributed across distant phyla and facilitate sporogenic differentiation of this bacterium. CIS loss results in decreases in extracellular DNA release, biomass accumulation, and spore formation in S. davawensis. CISs load an effector, which is a remote homolog of phage tapemeasure proteins, and its C-terminal domain has endonuclease activity responsible for the CIS-associated phenotypes. Our findings illustrate that CISs can contribute to the reproduction of bacteria through the action of the effector and suggest an evolutionary link between CIS effectors and viral cargos.
巻・号 15(1)
ページ 4442
公開日 2024-5-24
DOI 10.1038/s41467-024-48834-9
PII 10.1038/s41467-024-48834-9
PMID 38789435
PMC PMC11126660
MeSH Bacterial Proteins* / genetics Bacterial Proteins* / metabolism Bacteriophages* / genetics Bacteriophages* / physiology Phylogeny Spores, Bacterial* / metabolism Streptomyces* / virology Viral Proteins / genetics Viral Proteins / metabolism Viral Tail Proteins / genetics Viral Tail Proteins / metabolism
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