論文 - 詳細
| RRC ID | 84891 |
|---|---|
| 著者 | Johnson MD, Shepherd DC, Sakai HD, Mudaliyar M, Pandurangan AP, Short FL, Veith PD, Scott NE, Kurosawa N, Ghosal D. |
| タイトル | Cell-to-cell interactions revealed by cryo-tomography of a DPANN co-culture system. |
| ジャーナル | Nat Commun |
| Abstract |
DPANN is a widespread and diverse group of archaea characterized by their small size, reduced genome, limited metabolic pathways, and symbiotic existence. Known DPANN species are predominantly obligate ectosymbionts that depend on their host for proliferation. The structural and molecular details of host recognition, host-DPANN intercellular communication, and host adaptation in response to DPANN attachment remain unknown. Here, we use electron cryotomography (cryo-ET) to show that the Microcaldus variisymbioticus ARM-1 may interact with its host, Metallosphaera javensis AS-7 through intercellular proteinaceous nanotubes. Combining cryo-ET and sub-tomogram averaging, we show the in situ architectures of host and DPANN S-layers and the structures of the nanotubes in their primed and extended states. In addition, comparative proteomics and genomic analyses identified host proteomic changes in response to DPANN attachment. These results provide insights into the structural basis of host-DPANN communication and deepen our understanding of the host ectosymbiotic relationships. |
| 巻・号 | 15(1) |
| ページ | 7066 |
| 公開日 | 2024-8-16 |
| DOI | 10.1038/s41467-024-51159-2 |
| PII | 10.1038/s41467-024-51159-2 |
| PMID | 39152123 |
| PMC | PMC11329633 |
| MeSH | Archaea / genetics Archaea / metabolism Archaeal Proteins / genetics Archaeal Proteins / metabolism Cell Communication Coculture Techniques / methods Cryoelectron Microscopy* / methods Electron Microscope Tomography* / methods Nanotubes / chemistry Proteomics / methods Symbiosis* |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 14 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM33787 |