| RRC ID |
84899
|
| 著者 |
Ikei M, Miyazaki R, Monden K, Naito Y, Takeuchi A, Takahashi YS, Tanaka Y, Murata K, Mori T, Ichikawa M, Tsukazaki T.
|
| タイトル |
YeeD is an essential partner for YeeE-mediated thiosulfate uptake in bacteria and regulates thiosulfate ion decomposition.
|
| ジャーナル |
PLoS Biol
|
| Abstract |
Uptake of thiosulfate ions as an inorganic sulfur source from the environment is important for bacterial sulfur assimilation. Recently, a selective thiosulfate uptake pathway involving a membrane protein YeeE (TsuA) in Escherichia coli was characterized. YeeE-like proteins are conserved in some bacteria, archaea, and eukaryotes. However, the precise function of YeeE, along with its potential partner protein in the thiosulfate ion uptake pathway, remained unclear. Here, we assessed selective thiosulfate transport via Spirochaeta thermophila YeeE in vitro and characterized E. coli YeeD (TsuB) as an adjacent and essential protein for YeeE-mediated thiosulfate uptake in vivo. We further showed that S. thermophila YeeD possesses thiosulfate decomposition activity and that a conserved cysteine in YeeD was modified to several forms in the presence of thiosulfate. Finally, the crystal structures of S. thermophila YeeE-YeeD fusion proteins at 3.34-Å and 2.60-Å resolutions revealed their interactions. The association was evaluated by a binding assay using purified S. thermophila YeeE and YeeD. Based on these results, a model of the sophisticated uptake of thiosulfate ions by YeeE and YeeD is proposed.
|
| 巻・号 |
22(4)
|
| ページ |
e3002601
|
| 公開日 |
2024-4-1
|
| DOI |
10.1371/journal.pbio.3002601
|
| PII |
PBIOLOGY-D-24-00286
|
| PMID |
38656967
|
| PMC |
PMC11073785
|
| MeSH |
Amino Acid Sequence
Bacterial Proteins / genetics
Bacterial Proteins / metabolism
Biological Transport / genetics
Crystallography, X-Ray
Cysteine / metabolism
Escherichia coli* / genetics
Escherichia coli* / metabolism
Escherichia coli Proteins / genetics
Escherichia coli Proteins / metabolism
Membrane Proteins / genetics
Membrane Proteins / metabolism
Protein Binding
Sulfurtransferases* / genetics
Sulfurtransferases* / metabolism
Thiosulfates* / metabolism
|
| リソース情報 |
| 一般微生物 |
JCM20135 |
