Reference - Detail
| RRC ID | 84904 |
|---|---|
| Author | Ma JY, Yan Z, Sun XD, Jiang YQ, Duan JL, Feng LJ, Zhu FP, Liu XY, Xia PF, Yuan XZ. |
| Title | A hybrid photocatalytic system enables direct glucose utilization for methanogenesis. |
| Journal | Proc Natl Acad Sci U S A |
| Abstract |
Integration of methanogenic archaea with photocatalysts presents a sustainable solution for solar-driven methanogenesis. However, maximizing CH4 conversion efficiency remains challenging due to the intrinsic energy conservation and strictly restricted substrates of methanogenic archaea. Here, we report a solar-driven biotic-abiotic hybrid (biohybrid) system by incorporating cadmium sulfide (CdS) nanoparticles with a rationally designed methanogenic archaeon Methanosarcina acetivorans C2A, in which the glucose synergist protein and glucose kinase, an energy-efficient route for glucose transport and phosphorylation from Zymomonas mobilis, were implemented to facilitate nonnative substrate glucose for methanogenesis. We demonstrate that the photo-excited electrons facilitate membrane-bound electron transport chain, thereby augmenting the Na+ and H+ ion gradients across membrane to enhance adenosine triphosphate (ATP) synthesis. Additionally, this biohybrid system promotes the metabolism of pyruvate to acetyl coenzyme A (AcCoA) and inhibits the flow of AcCoA to the tricarboxylic acid (TCA) cycle, resulting in a 1.26-fold augmentation in CH4 production from glucose-derived carbon. Our results provide a unique strategy for enhancing methanogenesis through rational biohybrid design and reprogramming, which gives a promising avenue for sustainably manufacturing value-added chemicals. |
| Volume | 121(4) |
| Pages | e2317058121 |
| Published | 2024-1-23 |
| DOI | 10.1073/pnas.2317058121 |
| PMID | 38232281 |
| PMC | PMC10823229 |
| MeSH | Adenosine Triphosphate* / metabolism Biological Transport Electron Transport Energy Metabolism Methane* / metabolism Methanosarcina / metabolism |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | X(Twitter) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| General Microbes | JCM12185 |