| RRC ID |
84919
|
| Author |
Takei S, Teramoto K, Sekiguchi Y, Ihara H, Tohya M, Iwamoto S, Tanaka K, Khasawneh A, Horiuchi Y, Misawa S, Naito T, Kirikae T, Tada T, Tabe Y.
|
| Title |
Identification of Mycobacterium abscessus using the peaks of ribosomal protein L29, L30 and hemophore-related protein by MALDI-MS proteotyping.
|
| Journal |
Sci Rep
|
| Abstract |
Mycobacteroides (Mycobacterium) abscessus, which causes a variety of infectious diseases in humans, is becoming detected more frequently in clinical specimens as cases are spreading worldwide. Taxonomically, M. abscessus is composed of three subspecies of M. abscessus subsp. abscessus, M. abscessus subsp. bolletii, and M. abscessus subsp. massiliense, with different susceptibilities to macrolides. In order to identify rapidly these three subspecies, we determined useful biomarker proteins, including ribosomal protein L29, L30, and hemophore-related protein, for distinguishing the subspecies of M. abscessus using the matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) profiles. Thirty-three clinical strains of M. abscessus were correctly identified at the subspecies-level by the three biomarker protein peaks. This study ultimately demonstrates the potential of routine MALDI-MS-based laboratory methods for early identification and treatment for M. abscessus infections.
|
| Volume |
14(1)
|
| Pages |
11187
|
| Published |
2024-5-16
|
| DOI |
10.1038/s41598-024-61549-7
|
| PII |
10.1038/s41598-024-61549-7
|
| PMID |
38755267
|
| PMC |
PMC11099050
|
| MeSH |
Bacterial Proteins* / metabolism
Biomarkers / analysis
Biomarkers / metabolism
Humans
Mycobacterium Infections, Nontuberculous / diagnosis
Mycobacterium Infections, Nontuberculous / microbiology
Mycobacterium abscessus* / metabolism
Ribosomal Proteins* / analysis
Ribosomal Proteins* / metabolism
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization* / methods
|
| Resource |
| General Microbes |
JCM15297
JCM15300 |
