Reference - Detail
| RRC ID | 84919 |
|---|---|
| Author | Takei S, Teramoto K, Sekiguchi Y, Ihara H, Tohya M, Iwamoto S, Tanaka K, Khasawneh A, Horiuchi Y, Misawa S, Naito T, Kirikae T, Tada T, Tabe Y. |
| Title | Identification of Mycobacterium abscessus using the peaks of ribosomal protein L29, L30 and hemophore-related protein by MALDI-MS proteotyping. |
| Journal | Sci Rep |
| Abstract |
Mycobacteroides (Mycobacterium) abscessus, which causes a variety of infectious diseases in humans, is becoming detected more frequently in clinical specimens as cases are spreading worldwide. Taxonomically, M. abscessus is composed of three subspecies of M. abscessus subsp. abscessus, M. abscessus subsp. bolletii, and M. abscessus subsp. massiliense, with different susceptibilities to macrolides. In order to identify rapidly these three subspecies, we determined useful biomarker proteins, including ribosomal protein L29, L30, and hemophore-related protein, for distinguishing the subspecies of M. abscessus using the matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) profiles. Thirty-three clinical strains of M. abscessus were correctly identified at the subspecies-level by the three biomarker protein peaks. This study ultimately demonstrates the potential of routine MALDI-MS-based laboratory methods for early identification and treatment for M. abscessus infections. |
| Volume | 14(1) |
| Pages | 11187 |
| Published | 2024-5-16 |
| DOI | 10.1038/s41598-024-61549-7 |
| PII | 10.1038/s41598-024-61549-7 |
| PMID | 38755267 |
| PMC | PMC11099050 |
| MeSH | Bacterial Proteins* / metabolism Biomarkers / analysis Biomarkers / metabolism Humans Mycobacterium Infections, Nontuberculous / diagnosis Mycobacterium Infections, Nontuberculous / microbiology Mycobacterium abscessus* / metabolism Ribosomal Proteins* / analysis Ribosomal Proteins* / metabolism Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization* / methods |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| General Microbes | JCM15297 JCM15300 |