| RRC ID |
84920
|
| 著者 |
Park H, Yeo S, Ryu CB, Huh CS.
|
| タイトル |
A streamlined culturomics case study for the human gut microbiota research.
|
| ジャーナル |
Sci Rep
|
| Abstract |
Bacterial culturomics is a set of techniques to isolate and identify live bacteria from complex microbial ecosystems. Despite its potential to revolutionize microbiome research, bacterial culturomics has significant challenges when applied to human gut microbiome studies due to its labor-intensive nature. Therefore, we established a streamlined culturomics approach with minimal culture conditions for stool sample preincubation. We evaluated the suitability of non-selective medium candidates for maintaining microbial diversity during a 30-day incubation period based on 16S rRNA gene amplicon analysis. Subsequently, we applied four culture conditions (two preincubation media under an aerobic/anaerobic atmosphere) to isolate gut bacteria on a large scale from eight stool samples of healthy humans. We identified 8141 isolates, classified into 263 bacterial species, including 12 novel species candidates. Our analysis of cultivation efficiency revealed that seven days of aerobic and ten days of anaerobic incubation captured approximately 91% and 95% of the identified species within each condition, respectively, with a synergistic effect confirmed when selected preincubation media were combined. Moreover, our culturomics findings expanded the coverage of gut microbial diversity compared to 16S rRNA gene amplicon sequencing results. In conclusion, this study demonstrated the potential of a streamlined culturomics approach for the efficient isolation of gut bacteria from human stool samples. This approach might pave the way for the broader adoption of culturomics in human gut microbiome studies, ultimately leading to a more comprehensive understanding of this complex microbial ecosystem.
|
| 巻・号 |
14(1)
|
| ページ |
20361
|
| 公開日 |
2024-9-2
|
| DOI |
10.1038/s41598-024-71370-x
|
| PII |
10.1038/s41598-024-71370-x
|
| PMID |
39223323
|
| PMC |
PMC11368911
|
| MeSH |
Bacteria* / classification
Bacteria* / genetics
Bacteria* / isolation & purification
DNA, Bacterial / genetics
Feces* / microbiology
Gastrointestinal Microbiome* / genetics
Humans
RNA, Ribosomal, 16S* / genetics
|
| リソース情報 |
| 一般微生物 |
JCM36214
JCM36141
JCM36071
JCM36192
JCM36193
JCM36142
JCM36018
JCM36246
JCM36001
JCM36194
JCM36017
JCM36069 |
