| RRC ID |
84938
|
| 著者 |
Wu HC, Arima J, Kuan CH, Tsai YC, Lee YS, Chan CK, Chen YS.
|
| タイトル |
Bactofencin YH, a novel bacteriocin with high inhibitory activity against clinical Streptococcus species.
|
| ジャーナル |
World J Microbiol Biotechnol
|
| Abstract |
Strain Lactiplantibacillus plantarum D1 with bacteriocin producing ability was found in the intestine of Gambusia affinis. The bacteriocin was found to have high inhibitory activity against multiple Streptococcus species and several other Gram-positive and Gram-negative bacteria. Bacteriocin was purified from culture supernatant by ion-exchange chromatography, Sep-Pak C18 cartridge, and reverse-phase high-performance liquid chromatography (RP-HPLC). Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectral analysis determined that purified bacteriocin has a molecular mass of 2,731 Da. A partial N-terminal sequence KRKKHKXQIYNNGM was obtained from the Edman analysis. The N-terminal sequence was employed to search against a translation of the draft genome of strain D1. The translated full amino acid sequence of the mature peptide is as follows: NH2- KRKKHKCQIYNNGMPTGQYRWC, which has a molecular weight of 2738 Da. A BLAST search revealed that this bacteriocin was most similar to bactofencin A but differed from it with three amino acid residues. No identical peptide or protein has been previously reported, and this peptide, termed bactofencin YH, was therefore considered to be a new bacteriocin produced by Lactiplantibacillus plantarum D1.
|
| 巻・号 |
40(10)
|
| ページ |
290
|
| 公開日 |
2024-8-5
|
| DOI |
10.1007/s11274-024-04100-0
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| PII |
10.1007/s11274-024-04100-0
|
| PMID |
39102120
|
| MeSH |
Amino Acid Sequence*
Animals
Anti-Bacterial Agents* / chemistry
Anti-Bacterial Agents* / isolation & purification
Anti-Bacterial Agents* / pharmacology
Bacteriocins* / chemistry
Bacteriocins* / isolation & purification
Bacteriocins* / metabolism
Bacteriocins* / pharmacology
Chromatography, High Pressure Liquid
Gram-Negative Bacteria / drug effects
Gram-Positive Bacteria / drug effects
Microbial Sensitivity Tests
Molecular Weight*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Streptococcus* / drug effects
Streptococcus* / genetics
|
| リソース情報 |
| 一般微生物 |
JCM1157 |
