| RRC ID |
84958
|
| 著者 |
Aihara M, Gotoh Y, Shirahama S, Matsushima Y, Uchiumi T, Kang D, Hayashi T.
|
| タイトル |
Generation and maintenance of the circularized multimeric IS26-associated translocatable unit encoding multidrug resistance.
|
| ジャーナル |
Commun Biol
|
| Abstract |
In gram-negative bacteria, IS26 often exists in multidrug resistance (MDR) regions, forming a pseudocompound transposon (PCTn) that can be tandemly amplified. It also generates a circular intermediate called the "translocatable unit (TU)", but the TU has been detected only by PCR. Here, we demonstrate that in a Klebsiella pneumoniae MDR clone, mono- and multimeric forms of the TU were generated from the PCTn in a preexisting MDR plasmid where the inserted form of the TU was also tandemly amplified. The two modes of amplification were reproduced by culturing the original clone under antimicrobial selection pressure, and the amplified state was maintained in the absence of antibiotics. Mono- and multimeric forms of the circularized TU were generated in a RecA-dependent manner from the tandemly amplified TU, which can be generated in RecA-dependent and independent manners. These findings provide novel insights into the dynamic processes of genome amplification in bacteria.
|
| 巻・号 |
7(1)
|
| ページ |
597
|
| 公開日 |
2024-5-18
|
| DOI |
10.1038/s42003-024-06312-4
|
| PII |
10.1038/s42003-024-06312-4
|
| PMID |
38762617
|
| PMC |
PMC11102541
|
| MeSH |
Anti-Bacterial Agents / pharmacology
DNA Transposable Elements* / genetics
Drug Resistance, Multiple, Bacterial* / genetics
Klebsiella pneumoniae* / drug effects
Klebsiella pneumoniae* / genetics
Plasmids / genetics
Rec A Recombinases / genetics
Rec A Recombinases / metabolism
|
| IF |
4.165
|
| リソース情報 |
| 原核生物(大腸菌) |
JW2669
ME9062 |
