| RRC ID |
84960
|
| Author |
Bamford NC, Morris RJ, Prescott A, Murphy P, Erskine E, MacPhee CE, Stanley-Wall NR.
|
| Title |
TasA Fibre Interactions Are Necessary for Bacillus subtilis Biofilm Structure.
|
| Journal |
Mol Microbiol
|
| Abstract |
The extracellular matrix of biofilms provides crucial structural support to the community and protection from environmental perturbations. TasA, a key Bacillus subtilis biofilm matrix protein, forms both amyloid and non-amyloid fibrils. Non-amyloid TasA fibrils are formed via a strand-exchange mechanism, whereas the amyloid-like form involves non-specific self-assembly. We performed mutagenesis of the N-terminus to assess the role of non-amyloid fibrils in biofilm development. We find that the N-terminal tail is essential for the formation of structured biofilms, providing evidence that the strand-exchange fibrils are the active form in the biofilm matrix. Furthermore, we demonstrate that fibre formation alone is not sufficient to give structure to the biofilm. We build an interactome of TasA with other extracellular protein components, and identify important interaction sites. Our results provide insight into how protein-matrix interactions modulate biofilm development.
|
| Volume |
122(4)
|
| Pages |
598-609
|
| Published |
2024-10-1
|
| DOI |
10.1111/mmi.15315
|
| PMID |
39344640
|
| MeSH |
Amyloid / metabolism
Bacillus subtilis* / genetics
Bacillus subtilis* / metabolism
Bacillus subtilis* / physiology
Bacterial Proteins* / genetics
Bacterial Proteins* / metabolism
Biofilms* / growth & development
Extracellular Matrix / metabolism
|
| IF |
3.418
|
| Resource |
| Prokaryotes B. subtilis |
BKE05120
BKE09100
BKE35620
BKE35630
BKE39230
BKE25020
BKE03940 |
