論文 - 詳細
| RRC ID | 84966 |
|---|---|
| 著者 | Ikeda Y, Miyazaki R, Tsukazaki T, Akiyama Y, Mori H. |
| タイトル | Translation arrest cancellation of VemP, a secretion monitor in Vibrio, is regulated by multiple cis and trans factors, including SecY. |
| ジャーナル | J Biol Chem |
| Abstract |
VemP is a secretory protein in the Vibrio species that monitors cellular protein-transport activity through its translation arrest, allowing expression of the downstream secD2-secF2 genes in the same operon, which encode components of the protein translocation machinery. When cellular protein-transport function is fully active, secD2/F2 expression remains repressed as VemP translation arrest is canceled immediately. The VemP arrest cancellation occurs on the SecY/E/G translocon in a late stage in the translocation process and requires both trans factors, SecD/F and PpiD/YfgM, and a cis element, Arg-85 in VemP; however, the detailed molecular mechanism remains elusive. This study aimed to elucidate how VemP passing through SecY specifically monitors SecD/F function. Genetic and biochemical studies showed that SecY is involved in the VemP arrest cancellation and that the arrested VemP is stably associated with a specific site in the protein-conducting pore of SecY. VemP-Bla reporter analyses revealed that a short hydrophobic segment adjacent to Arg-85 plays a critical role in the regulated arrest cancellation with its hydrophobicity correlating with the stability of the VemP arrest. We identified Gln-65 and Pro-67 in VemP as novel elements important for the regulation. We propose a model for the regulation of the VemP arrest cancellation by multiple cis elements and trans factors with different roles. |
| 巻・号 | 300(10) |
| ページ | 107735 |
| 公開日 | 2024-10-1 |
| DOI | 10.1016/j.jbc.2024.107735 |
| PII | S0021-9258(24)02236-1 |
| PMID | 39233231 |
| PMC | PMC11470409 |
| MeSH | Bacterial Proteins* / genetics Bacterial Proteins* / metabolism Gene Expression Regulation, Bacterial* Protein Biosynthesis Protein Transport Vibrio / genetics Vibrio / metabolism Vibrio cholerae / genetics Vibrio cholerae / metabolism |
| IF | 4.238 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | ME9012 MC4100 |